中国南海海绵Haliclona baeri的化学成分研究  

Studies on chemical constituents of the sponge Haliclona baeri from the South China Sea

作　　者：王美霞[1,2] 肖艳芳 雷玉 徐石海 WANG Mei-xia;XIAO Yan-fang;LEI Yu;XU Shi-hai(Department of Chemical Engineering,Jieyang Polytechnic,Jieyang 522000,China;College of Chemistry and Materials,Jinan University,Guangzhou 511443,China)

机构地区：[1]揭阳职业技术学院化学工程系,广东揭阳522000 [2]暨南大学化学与材料学院,广东广州511443

出　　处：《中国海洋药物》2022年第3期79-83,共5页Chinese Journal of Marine Drugs

基　　金：广东省自然资源厅资助课题(粤自然资合[2021]48);国家自然科学基金项目(41876145,21672084);揭阳职业技术学院科研项目(2018JYCKY04)资助。

摘　　要：目的 对采自中国南海海域海绵Haliclona baeri 95%乙醇提取物的乙酸乙酯部位的化学成分进行研究。方法 运用薄层色谱、正相Silica gel柱色谱、SephadexLH-20柱色谱以及高效液相色谱(HPLC)等色谱分离手段,通过核磁共振谱(NMR)、质谱(MS)等波谱学方法,并结合文献报道,鉴定化合物的结构。结果 从海绵H. baeri中分离并鉴定了5个吲哚生物碱,分别为:(1H-indol-3-yl) oxoacetamide (1)、1H-indole-3-acetamide (2)、1H-indole-3-ethylethanoate (3)、1H-indole-3-carboxylic acid ethyl ester (4)和1H-indole-3-carboxylic acid (5)。结论从该属海绵中分离出化合物1~4。对化合物1进行生物活性测试,该化合物没有表现出明显的细胞毒、抗核因子κB、抗菌、抗病毒和抗污损活性。Objective To research the chemical components of the ethyl acetate fraction of the 95 % ethanol extract of the sponge Haliclona baeri collected from the South China Sea. Methods These compounds were separated and purified by modern chromatographic separation technology such as TLC, silica gel, Sephadex LH-20, High performance liquid chromatography(HPLC) and so on, and their structures were identified by the means of Nuclear magnetic resonance spectroscopy(NMR), Mass spectrometry(MS) and other spectroscopy methods, furthermore, by combaring with literature reports. Results Five indole alkaloids were separated and purified from the sponge H. baeri, those compounds were identified as(1H-indol-3-yl) oxoacetamide(1)、1H-indole-3-acetamide(2)、1H-indole-3-ethylethanoate(3)、1H-indole-3-carboxylic acid ethyl ester(4)and 1H-indole-3-carboxylic acid(5). Conclustion Compounds 1-4 have not been isolated from sponges of this genus. Compound 1 showed no obvious cytotoxic, anti-nuclear factor κB, antibacterial, antiviral and antifouling activities.

关 键 词：Haliclona baeri 化学成分 结构鉴定 吲哚生物碱 

分 类 号：O626.1[理学—有机化学] O629.3[理学—化学]