水产品中无机汞和甲基汞的液相色谱-原子荧光联用测定法  

Determination of inorganic mercury and methyl mercury in aquatic products by liquid chromatography-atomic fluorescence spectrometry

作　　者：杨婧[1] 胡迪[1] 范清 黄顺利[1] 赵清婷 YANG Jing;HU Di;FAN Qing;HUANG Shun-li;ZHAO Qing-ting(Physical and Chemical Department,Chongqing Yubei Center for Disease Control and Prevention,Chongqing,401120,China)

机构地区：[1]重庆市渝北区疾病预防控制中心理化科,重庆401120

出　　处：《职业与健康》2022年第12期1634-1636,1641,共4页Occupation and Health

摘　　要：目的建立测定水产品中汞形态(无机汞和甲基汞)的方法。方法以5 mol/L盐酸溶液超声水浴提取样品,样品中的甲基汞与L-半胱氨酸结合,经C18色谱柱分离,紫外消解将甲基汞还原为无机汞,由原子荧光光度计进行测定。结果无机汞的检出限为0.28μg/L,甲基汞的检出限为0.36μg/L,回收率为80.8%~90.0%,相对标准偏差在3.5%~5.0%之间,相关系数r≥0.999,符合相关国家标准。结论所建立的液相色谱-原子荧光联用法操作简便、快速、回收率高,适用于水产品中的汞形态分析。Objective To develop a method for the determination of mercury form(inorganic and methyl mercury)in aquatic products by liquid chromatography with atomic fluorescence spectrometry.Methods Samples were extracted by ultrasonic water bath with 5 mol/L hydrochloric acid solution,methyl mercury in the samples combined with L-cysteine and the sample solution was separated on a C18 chromatographic column.The methyl mercury was reduced to inorganic mercury by ultraviolet digestion,then was determined by atomic fluorescence photometer.Results The detection limit for norganic mercury was 0.28μg/L,and that for methyl mercury was 0.36μg/L.The recovery rates were 80.8%-90.0%,the relative standard deviation was 3.5%-5.0%,with the correlation coefficient(r)≥0.999,which met the relevant national standards.Conclusion The method has the advantages of convenient operation,high speed and high recovery rate,and it is suitable for the analysis of mercury speciation in aquatic products.

关 键 词：液相色谱 原子荧光 甲基汞 无机汞 

分 类 号：R115[医药卫生—公共卫生与预防医学]