Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses  

作　　者：Wu Liu Yuyun Zhang Xing Fang Sorrel Tran Ning Zhai Zhengfei Yang Fu Guo Lyuqin Chen Jie Yu Madalene SIson Teng Zhang Lijun Sun Hongwu Bian Yijing Zhang Li Yang Lin Xu 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,300 Fenglin Road,Shanghai 200032,China [2]University of Chinese Academy of Sciences,19A Yuquan Road,Beijing,100049,China [3]Department of Plant Pathology,University of Georgia,Athens,GA 30602,USA [4]College of Life Sciences,Shanghai Normal University,Shanghai 200234,China [5]Hainan Institute of Zhejiang University,Yazhou Bay Science and Technology City,Sanya 572025,China [6]School of Life Sciences,Nantong University,Nantong,China [7]Institute of Genetic and Regenerative Biology,Key Laboratory for Cell and Gene Engineering of Zhejiang Province,College of Life Sciences,Zhejiang University,Hangzhou 310058,China [8]State Key Laboratory of Genetic Engineering,Collaborative Innovation Center of Genetics and Development,Department of Biochemistry,Institute of Plant Biology,School of Life Sciences,Fudan University,Shanghai 200438,China [9]These authors contributed equally to this article [10]Department of Pharmacology and Chemical Biology,UPMC Hillman Cancer Center,Magee-Womens Research Institute,University of Pittsburgh,Pittsburgh,PA,USA

出　　处：《Plant Communications》2022年第4期83-104,共22页植物通讯（英文）

基　　金：supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDB27030103);the National Natural Science Foundation of China(32000175/31770285/32070397);the Youth Innovation Promotion Association CAS(2014241);the Chinese Academy of Sciences.A portion of this research was supported by a Global Research Collaboration Grant from the Offices of Research and Global Engagement to L.Y.from the University of Georgia and National Science Foundation under Grant NO.IOS2039313 to L.Y.

摘　　要：Detached Arabidopsis thaliana leaves can regenerate adventitious roots,providing a platformfor studying de novo root regeneration(DNRR).However,the comprehensive transcriptional framework of DNRR remains elusive.Here,we provide a high-resolution landscape of transcriptome reprogramming from wound response to root organogenesis in DNRR and show key factors involved in DNRR.Time-lapse RNA sequencing(RNA-seq)of the entire leaf within 12 h of leaf detachment revealed rapid activation of jasmonate,ethylene,and reactive oxygen species(ROS)pathways in response towounding.Genetic analyses confirmed that ethylene andROSmay serve as wound signals to promoteDNRR.Next,time-lapse RNA-seq within 5 d of leaf detachment revealed the activation of genes involved in organogenesis,wound-induced regeneration,and resource allocation in the wounded region of detached leaves during adventitious rooting.Genetic studies showed that BLADE-ON-PETIOLE1/2,which control aboveground organs,PLETHORA3/5/7,which control root organogenesis,and ETHYLENE RESPONSE FACTOR115,which controlswound-induced regeneration,are involved in DNRR.Furthermore,single-cell RNA-seq data revealed gene expression patterns in thewounded region of detached leaves during adventitious rooting.Overall,our study not only provides transcriptome tools but also reveals key factors involved in DNRR from detached Arabidopsis leaves.

关 键 词：single-cell RNA-seq time-lapse RNA-seq de novo root regeneration plant regeneration WOUNDING Arabidopsis thaliana 

分 类 号：Q94[生物学—植物学]