A chromosome-level genome assembly of the blackspotted croaker(Protonibea diacanthus)  

作　　者：Tianjun Xu Ye Li Weiwei Zheng Yuena Sun 

机构地区：[1]Laboratory of Fish Molecular Immunology,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai,201306,China [2]Laboratory of Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,266200,China [3]Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University),Ministry of Education,Shanghai,201306,China [4]National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai,201306,China

出　　处：《Aquaculture and Fisheries》2022年第6期616-622,共7页渔业学报（英文）

基　　金：the National Key Research and Development Project(2018YFD0900301);the National Natural Science Foundation of China(31802325).

摘　　要：The blackspotted croaker(Protonibea diacanthus)is an endangered coastal marine fish.It is also a valuable species that is cultured on the southeast coast of China.While some genetic studies have been conducted to protect this species,genomic resources are lacking.Here,we report a chromosome-scale assembly of P.diacanthus genome by high-depth genome sequencing,assembly,and annotation.The genome scale was 635.69 Mb with contig and scaffold N50 length of 3.33 Mb and 25.60 Mb,respectively.Hi-C scaffolding of the genome resulted in 24 chromosomes of 94.15%total genome.We predicted 23,971 protein-coding genes.In addition,we constructed a phylogenetic tree using 2755 single-copy gene families and identified 462 unique gene families in P.diacanthus genome compared to three other sciaenids.What’s more,from the analysis of gene families,we found that several gene families related to innate immunity were significantly expanded in the blackspotted croaker genome compared to other teleost genomes.The high-quality genome can improve our understanding of the molecular mechanisms behind economically valuable traits and provide insights into characteristics of the immune system.

关 键 词：Blackspotted croaker Chromosomal assembly Genome annotation Genome evolution PHYLOGENETIC 

分 类 号：S94[农业科学—水产养殖]