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作 者:Xiongkun Zhang Shan Wu Xiaojie Sun Monika Mortimer Yichao Wu Ming Zhang Qiaoyun Huang Peng Cai
机构地区:[1]State Key Laboratory of Agricultural Microbiology,College of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,China [2]Institute of Environmental and Health Sciences,College of Quality and Safety Engineering,China Jiliang University,Hangzhou 310018,China
出 处:《Soil Ecology Letters》2022年第3期213-223,共11页土壤生态学快报(英文)
基 金:the National Natural Science Foundation of China(41877029,41961130383);Royal SocietyNewton Advanced Fellowship(NAF\R1\191017);Wuhan Science and Technology Bureau(2019020701011469).
摘 要:Microfluidics confers unique advantages in microbiological studies as these devices can accurately replicate the micro-and even nano-scale structures of soil to simulate the habitats of bacteria.It not only helps us understand the spatial distribution of bacterial communities(such as biofilms),but also provides mechanistic insights into microbial behaviors including chemotaxis and horizontal gene transfer(HGT).Microfluidics provides a feasible means for real-time,in situ studies and enables indepth exploration of the mechanisms of interactions in the soil microbiome.This review aims to introduce the basic principles of microfluidic technology and summarize the recent progress in microfluidic devices to study bacterial spatial distribution and functions,as well as biological processes,such bacterial chemotaxis,biofilm streamers(BS),quorum sensing(QS),and HGT.The challenges in and future development of microfluidics for soil microbiological studies are also discussed.
关 键 词:MICROFLUIDICS Soil bacteria Biofilms CHEMOTAXIS Quorum sensing Horizontal gene transfer
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