Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes  

作　　者：Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu Baohui Liu Fanjiang Kong 

机构地区：[1]Innovative Center of Molecular Genetics and Evolution,School of Life Sciences,Guangzhou University,Guangzhou 510405,China [2]College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163000,China [3]Department of Agriculture,Northeast Agricultural University,Harbin 150000,China [4]Soybean Research Institute,National Engineering Research Center for Soybean,Jilin Academy of Agricultural Sciences,Changchun 130033,China [5]National Center for Soybean Improvement,National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China [6]The Innovative Academy of Seed Design,Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China

出　　处：《Molecular Plant》2022年第2期308-321,共14页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China(grant nos.32090065 and 32001508 to L.D.,32090064 and 31725021 to F.K.,31930083 to B.L,31901568 to Q.C,32022062 to S.Lu.,32001502 to Y,Z);and also supported by the Major Program of Guangdong Basic and Applied FResearch(grant no.2019B030302006 to F.K.andB.L).

摘　　要：Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication.

关 键 词：soybean WILD natural selection artificially selection latitude adaptation flowering time 

分 类 号：S565.1[农业科学—作物学] Q94[生物学—植物学]