Hormesis效应带剂量镉对HKC中EMT相关因子表达的影响  

Effects of Cd at the Dose of Hormetic Zone on the Expression of EMT-related Factors in HKC

作　　者：吴帆 陈淼 甄旭斌 黄煌涛 马贞初 邹志辉 Wu Fan;Chen Miao;Zhen Xubin;Huang Huangtao;Ma Zhenchu;Zou Zhihui(School of Public Health Guangdong Pharmaceutical University,Guangzhou 510310,China)

机构地区：[1]广东药科大学公共卫生学院,广东广州510310

出　　处：《广东化工》2022年第12期11-13,共3页Guangdong Chemical Industry

基　　金：2018年度佛山市自筹经费类科技计划项目(医学类科技攻关)(K682819012012);2019年广东省大学生创新创业训练计划项目。

摘　　要：研究Hormesis效应带剂量(0.625~2.5μM)镉对人肾小管上皮HKC细胞中EMT相关因子表达的影响。将对数生长期HKC细胞分别暴露于终浓度为0(对照)、0.625、1.25、2.5μM的氯化镉中,连续染毒14 d,采用qRT-PCR检测不同剂量镉对c-myc,cyclin D1,α-SMA mRNA表达水平的影响,采用Western blot技术检测不同剂量镉对TGF-β1和Wnt蛋白表达的影响。一定剂量镉可显著上调HKC细胞中cyclinD1、c-myc、α-SMA mRNA的表达,Hormesis效应带剂量镉暴露可显著激活TGF-β1的表达呈明显的剂量效应。Hormesis效应带剂量镉亚急性暴露可促进肾小管上皮细胞中α-SMA和TGF-β1等EMT相关因子的表达。To explore the effects of Cd at the dose of Hormetic Zone on the expression of EMT-related factors in HKC.The HKC cells at exponential growth phase were exposed to 0(control),0.625,1.25 and 2.5 μM cadmium chloride for 14 d.The effects of Cd on expression of cyclinD1,c-myc and α-SMA mRNA was determined by qRT-PCR assay.And the effects of Cd on expression of TGF-β1 and Wnt was determined by Western Blot assay.Cd at a certain dosage could obviously induce the expression of cyclinD1,c-myc,α-SMA mRNA and enhance the expressions of TGF-β1 in a dose effect.The subacute exposure of Cd at the dose of Hormetic Zone can activate the expression of EMT-related factors including α-SMA and TGF-β1 in HKC.

关 键 词：WNT/Β-CATENIN TGF-Β1 EMT样转变 镉 Hormesis效应带剂量 

分 类 号：R181[医药卫生—流行病学]