基于称重法的7个烟草品种旺长期抗旱性评价  

Evaluation of Drought Resistance of Tobacco Varieties at Vigorous Stage Based on Weighing Method

作　　者：汪志威 胡秋舲 祖达 刘仁祥[3] WANG Zhi-wei;HU Qiu-ling;ZU Da(Liupanshui Academy of Agricultural Sciences,Liupanshui,Guizhou 553000;Liupanshui Bureau of Agriculture and Rural Areas,Liupanshui,Guizhou 553000)

机构地区：[1]六盘水市农业科学研究院,贵州六盘水553000 [2]六盘水市农业农村局,贵州六盘水553000 [3]贵州大学,贵州贵阳550025

出　　处：《安徽农业科学》2022年第14期25-30,共6页Journal of Anhui Agricultural Sciences

基　　金：贵州省高层次创新型人才培养计划项目(黔科合平台人才〔2016〕5663);贵州省烟草公司科技项目(201602,201904,2020XM07)。

摘　　要：为形成一种烟草旺长期的快速准确的抗旱鉴定方法,对7分烟草品种进行抗旱性鉴定。以生产常用的7个烟草品种为试验材料,采用盆栽方法,在旺长期用称重法进行连续干旱(土壤相对含水量为30%~35%)处理21 d,比较叶片MDA和脯氨酸含量、SOD和CAT活性变化,并以其为联合筛选指标,以隶属函数法对7份材料进行抗旱性评价。结果表明,与对照相比,连续干旱14 d后干旱敏感品种TN90和红花大金元MDA含量分别显著或极显著升高45.5%、27.1%,脯氨酸含量分别极显著增长58.68%、35.6%,SOD、CAT活性显著或极显著降低;高抗品种K326和南江三号的MDA含量分别降低12.32%、23.91%,脯氨酸含量分别极显著升高115.18%、92.74%,SOD和CAT活性显著或极显著增强。连续干旱处理21 d后,干旱敏感品种TN90和红花大金元MDA含量分别较对照显著或极显著升高28.94%、25.81%,TN90的脯氨酸含量极显著增长34.45%,SOD、CAT活性显著降低;高抗品种K326和南江三号的脯氨酸含量分别极显著增加85.51%、96.21%,SOD和CAT活性均显著增强。因此,MDA和脯氨酸含量、SOD和CAT活性可以作为烟草旺长期的抗旱性联合鉴定指标,以称重法控制土壤含水量,采用隶属函数法对材料进行抗旱性评价是一种较快速的烟草抗旱鉴定方法,通过该方法筛选出2个干旱敏感品种TN90和红花大金元和筛选出2个高抗品种K326和南江三号。To form a rapid and suitable drought-tolerant screening method at vigorous stage of tobacco,7 varieties commonly used for production were selected as materials,pot experiment method was adopted whih continuous drought treatment(soil relative water content was 30%-35%)for 21 d at vigorous stage by weighting method.The leaf MDA and proline contents and SOD and CAT activities change were tested,which were chosen to be the joint selection index for screening the varieties.Compared with the control after 14 d drought treatment,the MDA content of drought-sensitive cultivars TN90 and Honghuadajinyuan were significantly and extremely significantly increased by 45.5%and 27.1%,respectively.Proline content was significantly increased by 58.68%and 35.6%compared with the control.MDA contents of drought-sensitive cultivars TN90 and Honghuadajinyuan were significantly increased by 45.5%and 27.1%,respectively,SOD and CAT activities were significantly or extremely significantly decreased.The MDA contents of K326 and Nanjiang 3 were significantly decreased by 12.32%and 23.91%,the MDA contents of drought-sensitive cultivars TN90 and Honghuadajinyuan were significantly increased by 45.5%and 27.1%respectively,proline content was significantly increased by 115.18%and 92.74%respectively,compared with the control after 14 d drought treatment.After 21 d continuous drought treatment,the MDA content of drought-sensitive cultivars TN90 and Honghuadajinyuan were significantly increased by 28.94%and 25.81%respectively,proline content of TN90 was significantly increased by 34.45%,SOD and CAT activities were significantly decreased.The proline content of K326 and Nanjiang 3 were significantly increased by 85.51%and 96.21%respectively,and SOD and CAT activities were significantly increased.As a result,MDA and proline contents,SOD and CAT activities could be used as a tobacco prosperous joint appraisal indexes of drought resistance of tobacco in vigorous stage,by weighing method to control soil moisture content,using subordinate function method t

关 键 词：烟草 抗旱评价 MDA 脯氨酸 SOD CAT 

分 类 号：S572[农业科学—烟草工业]