紫花苜蓿品种苗期耐盐性分析及评价指标筛选  

Analysis of Salinity-Tolerance and Screening Evaluation Indicators to Salinity-Tolerance in Medicago Sativa Seedlings

作　　者：余如刚[1] 王雪茄 王国良[2] 徐智明 高晴晴[1] 杜雪玲 张宇龙[3] YU Ru-gang;WANG Xue-jia;WANG Guo-liang;XU Zhi-ming;GAO Qing-qing;DU Xue-ling;ZHANG Yu-long(College of Life Sciences, Huaibei Normal University, Huaibei, Anhui Province 235000, China;Institute of Leisure Agriculture, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province 250010, China;Purple pasture Co., Ltd, Wuhe, Anhui Province 233300, China;Anhui Academy of Agricultural Sciences, Hefei, Anhui Province 230031, China)

机构地区：[1]淮北师范大学生命科学学院,安徽淮北235000 [2]山东省农业科学院休闲农业研究所,山东济南250010 [3]秋实草业有限公司,安徽五河233300 [4]安徽省农业科学院,安徽合肥230031

出　　处：《草地学报》2022年第7期1781-1789,共9页Acta Agrestia Sinica

基　　金：安徽省重点研究与开发计划项目(202004a06020046);财政部和农业农村部:国家现代农业产业技术体系;山东省农业重大应用技术创新项目(SD2019XM001);国家级大学生创新创业训练计划项目(202110373019)资助。

摘　　要：为了探讨紫花苜蓿苗期耐盐特性,筛选耐盐性快速评价指标并建立耐盐性数学评价模型,本研究以20个紫花苜蓿品种为材料,采用盆栽沙培法,设置两个NaCl处理水平:0和150 mmol·L-1,胁迫5周后测定各品种的17个形态和生理指标,以各单项指标的耐盐系数为基础,运用主成分分析、隶属函数值法和逐步回归分析等方法进行综合评价。结果显示:将17个单项指标转换为5个相互独立的综合指标,累计贡献率为87.07%;获得各品种综合耐盐评价值(D值),对D值进行聚类分析,筛选出1个高度耐盐、7个耐盐、4个中度耐盐、6个盐敏感和2个盐高敏感品种。建立紫花苜蓿苗期耐盐性评价数学模型:D′=-0.284+1.342 ADW+0.182 LN(R2=0.967,P=0.001),并筛选出地上部干重和单株叶片数为紫花苜蓿苗期耐盐性评价指标。The experiment aim is to explore the salinity tolerance of different Medicago sativa L.seedlings,screen suitable salinity tolerance evaluation indicators,and to establish a mathematical evaluation model for salinity tolerance.Using potted sand culture method,17 morphological and physiological indicators of 20 M.sativa varieties were determined with 0 and 150 mmol·L-1 NaCl treatments for five weeks in this study.Based on the salt-tolerant coefficient of each single indicator,a comprehensive evaluation was performed by principal components analysis,membership function method and stepwise regression analysis.The results showed that the 17 single indices were transformed into five independent comprehensive indices.The cumulative contribution reached 87.07%.Then,the comprehensive salinity tolerance evaluation value(D value)was obtained and used to cluster analysis,and one highly salt-tolerant,seven salt-tolerant,four moderately salt-tolerant,six salt-sensitive and two highly salt-sensitive M.sativa L.varieties were screened out.Finally,a mathematical model for the evaluation of M.sativa L.salt tolerance was established,D′=-0.284+1.342 ADW+0.182 LN(R2=0.967,P=0.001),and the aboveground dry weight and leaf number per plant indices were selected as indicators to evaluate the salt tolerance of M.sativa L.seedling.

关 键 词：紫花苜蓿 苗期 耐盐性 综合评价 

分 类 号：S541[农业科学—作物学]