基于UPLC-QTOF-MS技术鉴别10种龟鳖甲类中药的真伪  

Authenticity identification of 10 kinds of Testudinis Carapax et Plastrum and Trionycis Carapax based on UPLC-QTOF-MS

作　　者：钱敏 刘宇文[2] 刘晓华 王云飞 倪承前 蔡伟 QIAN Min;LIU Yu-wen;LIU Xiao-hua;WANG Yun-fei;NI Cheng-qian;CAI Wei(Zhejiang Chinese Medical University Medical Pieces Co.,Limited,Hangzhou 311401,China;Hangzhou Institute for Food and Drug Control,Hangzhou 310022,China;Department of Chinese Materia Medica,Zhejiang Pharmaceutical College,Ningbo 315500,China)

机构地区：[1]浙江中医药大学中药饮片有限公司,杭州311401 [2]杭州市食品药品检验研究院,杭州310022 [3]浙江药科职业大学中药学院,宁波315500

出　　处：《中华中医药杂志》2022年第6期3434-3440,共7页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省基础公益研究项目(No.L G F21H280006);浙江省食品药品监督管理局科技计划项目(No.2019009);浙江省医药卫生科技项目(No.2015KYA065);浙江省中医药科技计划项目(No.2015ZB104)。

摘　　要：目的:建立专属性的龟鳖甲类中药的鉴别真伪方法。方法:借鉴胶类药材特征肽鉴别方法,使用超高效液相色谱-四级杆飞行时间质谱(UPLC-QTOF-MS)结合化学统计方法分析酶解样品溶液。结果:筛选出乌龟、巴西龟、花龟、黄喉拟水龟、黄缘闭壳龟、缅甸陆龟、小鳄龟、印度棱背龟、鳖、佛罗里达鳖10种龟鳖类正伪品甲壳的特征肽,获得主要二级碎片离子,并初步测定序列,获得的特征离子对10种龟鳖甲类正伪品的专属性鉴别效果较好。结论:该方法可作为一种快速准确鉴别龟鳖甲正伪品的鉴定方法。Objective:To establish a specificity authenticity identification method for Testudinis Carapax et Plastrum and Trionycis Carapax.Methods:This study used the identification method of characteristic peptides of gelatins for reference,and used the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)combined with chemical statistical method for analysis.Results:The characteristic peptide of carapax or plastrum of Chinemys reevesii(Grsy),Trachemys scripta elegans(Wied.),Ocadia sinensis(Gray),Clemmys mutica(Cantor),Cyclemys flavomarginata Gray.,Testudo elongate(Blyth),Kachuga tectum(Gray),Chelydra serpentina(Linnaeus),Trionyx sinensis Wiegmann,Apalone ferox(Schneider)were screened out,and the main secondary fragment ions were obtained,and the obtained characteristic ions had a good effect on the identification of 10 kinds of tortoise shells and turtle shells.Conclusion:This method can be used as a rapid and accurate method to identify the authenticate of Testudinis Carapax et Plastrum and Trionycis Carapax.

关 键 词：龟甲 鳖甲 混淆品 特征离子 超高效液相色谱-串联四级杆飞行时间质谱 真伪鉴别 特征肽 专属性 

分 类 号：R282.5[医药卫生—中药学]