电针对肥胖大鼠白色脂肪组织PGC-1α/Irisin/UCP1信号通路的影响  

Effects of electroacupuncture on PGC-1α/Irisin/UCP1 signaling pathway in white adipose tissue of obese rats

作　　者：张艳佶 黄伟[2,3] 李佳 徐蔻 张英溶 夏鸿杰 周仲瑜 ZHANG Yan-ji;HUANG Wei;LI Jia;XU Kou;ZHANG Ying-rong;XIA Hong-jie;ZHOU Zhong-yu(College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430065,China;Department of Acupuncture,Hubei Provincial Hospital of TCM,Wuhan 430061,China;Department of Acupuncture,Affiliated Hospital of Hubei University of Chinese Medicine,Wuhan 430061,China)

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430065 [2]湖北省中医院针灸科,武汉430061 [3]湖北中医药大学附属医院针灸科,武汉430061

出　　处：《中华中医药杂志》2022年第6期3471-3474,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖北省第二届医学领军人才工程培养对象暨湖北名医工作室项目(No.鄂卫通[2019]47号);国家自然科学基金项目(No.81674081);湖北省中医院首批昙华林名医、学子培养项目(No.鄂中医院[2018]72号);国家中医药管理局岐黄工程项目(No.国中医药人教函[2018]284号);2019年国家中医药管理局《中医药循证能力建设项目》(No.2019XZZX-ZJ006);2020年针灸治未病湖北省协同创新中心项目(No.HBPCIC-2020-05);湖北中医名师工作室项目(No.卫生计生通报[2018]15号)。

摘　　要：目的:观察电针对高脂饮食诱导的肥胖(DIO)大鼠白色脂肪组织(WAT)过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、鸢尾素(Irisin)、解偶联蛋白-1(UCP1)信号通路的影响。方法:将65只SPF级雄性SD大鼠随机分为普食组(10只)和高脂组(55只)。采用高脂饮食喂养制造肥胖大鼠模型。高脂组中成功造模的32只肥胖大鼠随机分为模型组、电针组、电针+AAV-VC组、电针+AAV-PGC-1α组,每组8只。每周测量各组大鼠体质量。采用qPCR法检测WAT中PGC-1α、FNDC5、UCP1 mRNA表达。结果:与对照组比较,模型组治疗后体质量显著增高(P<0.05);PGC-1α、FNDC5、UCP1 mRNA表达显著降低(P<0.05);与模型组比较,电针组、电针+AAV-VC组体质量显著降低(P<0.05);PGC-1α、FNDC5、UCP1 mRNA表达显著升高(P<0.05);而电针+AAV-PGC-1α组体质量并未下降,PGC-1α、FNDC5、UCP1 mRNA表达水平也并未上调。结论:电针可促进DIO大鼠白色脂肪中PGC-1α、Irisin、UCP1表达,提示电针减肥可能是通过PGC-1α/Irisin/UCP1信号通路以促进白色脂肪棕色化来实现的。Objective:To investigate the effects of electroacupuncture(EA)on the signaling pathways of peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α),Irisin,and uncoupling protein-1(UCP1)in the white adipose tissue(WAT)of diet-induced obese(DIO)rats.Methods:Sixty-five male SD rats were randomly divided into normal diet group(control group,n=10)and high-fat diet group(n=55).A high-fat diet was adopted to make DIO models.In the high fat diet group,32 successful model rats were randomly divided into four groups:model group,EA group,EA+adeno-associated virus-vacant(AAV-VC)group,EA+adeno-associated virus-PGC-1α(AAV-PGC-1α)group,8 rats in each group.The body mass of each group of rats was measured every week.qPCR method was applied to detect the mRNA levels of PGC-1α,FNDC5,UCP1 in WAT.Results:Compared with the control group,the body mass of the model group was significantly higher(P<0.05),and the mRNA expression of PGC-1α,FNDC5 and UCP1 were significantly lower(P<0.05).Compared with the model group,the body mass of the EA group and the EA+AAV-VC group was significantly lower(P<0.05),and the mRNA expressions of PGC-1α,FNDC5 and UCP1 were significantly higher(P<0.05),while the body mass of the EA+AAV-PGC-1αgroup did not significantly decrease,the mRNA expression levels of PGC-1α,FNDC5,and UCP1 in the EA+AAV-PGC-1αgroup were also not upregulated.Conclusion:EA can significantly promote the expression of PGC-1α,Irisin,and UCP1 in WAT of DIO rats,it suggests that the effect of EA therapy to promote the browning of white adipose tissue may be achieved through the PGC-1α/Irisin/UCP1 signaling pathway.

关 键 词：电针 肥胖 过氧化物酶体增殖物激活受体γ辅激活因子1α 解偶联蛋白-1 鸢尾素 机制 动物模型 白色脂肪组织 

分 类 号：R245.97[医药卫生—针灸推拿学]