异类叶升麻苷和桔梗皂苷D的体外抗肺癌研究  

Study on the inhibitory effect of the isoverbascoside and platycodin D on anti-lung cancer in vitro

作　　者：余娜 王芬[2] 周厚元 陈巧巧 刘宁[2] 谢壮鑫 唐皓[1] 朱克俭[1] 李顺祥[2] YU Na;WANG Fen;ZHOU Hou-yuan;CHEN Qiao-qiao;LIU Ning;XIE Zhuang-xin;TANG Hao;ZHU Ke-jian;LI Shun-xiang(Hunan Academy of Chinese Medicine,Changsha 410006,China;Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南省中医药研究院,长沙410006 [2]湖南中医药大学,长沙410208

出　　处：《中华中医药杂志》2022年第6期3510-3514,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学基金项目(No.2020JJ5428);湖南省中医药管理局课题(No.201935);中药学一流学科,湖南省普通高等学校中药现代化研究重点实验室。

摘　　要：目的:探讨异类叶升麻苷与桔梗皂苷D单用及配伍对A549细胞增殖、迁移和侵袭的影响及作用机制。方法:采用CCK-8、细胞划痕、Transwell试验分别检测细胞增殖、迁移和侵袭,Western Blot检测EphA2/AKT/mTOR通路蛋白,ELISA检测肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)含量。结果:异类叶升麻苷和桔梗皂苷D均能抑制细胞增殖,且IC50分别为141、170μg/mL。与对照组比较,桔梗组、配伍组愈合率显著下降(P<0.01),给药各组穿膜细胞数均显著减少(P<0.01),其中配伍组最为明显。与对照组比较,桔梗组、配伍组显著下调EphA2、p-EphA2蛋白表达(P<0.01),桔梗组显著下调p-AKT、mTOR蛋白表达(P<0.01)。与对照组比较,桔梗组IL-6、TNF-α显著升高(P<0.01,P<0.05),而异类叶组TNF-α和配伍组IL-6显著下调(P<0.01)。结论:异类叶升麻苷、桔梗皂苷D通过调控EphA2/AKT/mTOR通路和调节TNF-α、IL-6抑制A549细胞增殖、迁移和侵袭。且二者配伍显著调控Eph A2因子,并能减轻桔梗诱导IL-6和TNF-α的上调,推测配伍组可能具有增效减毒的作用。Objective:To explore the effects and mechanism of the two components of isoverbascoside and platycodin D(PD)on the proliferation,migration and invasion of A549 cells.Methods:Proliferation,migration and invasion of cells were detected by CCK-8,cell scratch and Transwell assay,EphA2/AKT/mTOR pathway protein expression was detected by Western Blot,and TNF-αand IL-6 content was detected by ELISA.Results:Isoverbascoside and PD could inhibit cell proliferation with respectively IC50 of 141 and 170μg/mL.Compared with the control group,the healing rate of the PD group and the mixed group decreased significantly(P<0.01),the number of transmembrane cells in each group was significantly decreased(P<0.01),and the mixed group was the most significant.Compared with the control group,the EphA2 and p-EphA2 proteins were significantly down-regulated in PD group and mixed group(P<0.01),p-Akt and mTOR protein was significantly down-regulated in PD group(P<0.01).Compared with the control group,TNF-αand IL-6 increased in PD group(P<0.01,P<0.05),while TNF-αin the isoverbascoside group and IL-6 in the mixed group were significantly down-regulated(P<0.01).Conclusion:Isoacteoside and PD can inhibit the migration and invasion of A549 cells by regulating EphA2/AKT/mTOR pathway and regulating TNF-αand IL-6.In addition,the mixed group significantly regulates EphA2 factor,and can reduce the increase of IL-6 and TNF-αof PD.It is speculated that the mixed group may have the effect of increasing efficiency and reducing toxicity.

关 键 词：异类叶升麻苷 桔梗皂苷D 抗肿瘤 非小细胞肺癌 EphA2/AKT/mTOR 肿瘤坏死因子-α 白介素-6 增效减毒 

分 类 号：R285[医药卫生—中药学]