11β-HSDl在非酒精性脂肪性肝病大鼠肝脏中的表达及大黄素的干预作用  

Expression of 11β-HSDl in liver of rat with non-alcoholic fatty liver disease and the intervention effects of emodin

作　　者：孙红爽[1] 李鹏霖 刘永双 乜春城[1] 高玲娜 Sun Hongshuang;Li Penglin;Liu Yongshuang;Nie Chuncheng;Gao Lingna(Department of Pharmacy,Harrison International Peace Hospital,Hengshui 053000,Hebei Province,China)

机构地区：[1]哈励逊国际和平医院药学部,河北衡水053000

出　　处：《中国肝脏病杂志（电子版）》2022年第2期11-17,共7页Chinese Journal of Liver Diseases：Electronic Version

基　　金：河北省2021年度医学科学研究课题(20211184)。

摘　　要：目的探讨11β-羟基类固醇脱氢酶1(11β-hydroxysteroid dehydrogenase type 1,11β-HSDl)在非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)大鼠肝脏中的表达及大黄素的干预作用。方法将30只雄性Wistar大鼠随机分为对照组、模型组和治疗组,每组10只。对照组给予普通饲料,模型组和治疗组喂以自制高脂饲料,从第9周开始治疗组给予大黄素干预治疗,第14周末实验结束。计算各组大鼠肝指数,检测血液和肝组织生物化学指标,包括丙氨酸氨基转移酶(alaninne aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、总胆固醇(totalcholesterol,TG)、甘油三酯(triglycerides,TC)、高密度脂蛋白(high density lipoprotein,HDL)、游离脂肪酸(free fatty acid,FFA)及血清肿瘤坏死因子-α(tumor necrosis factorα,TNF-α),采用实时荧光定量聚合酶链式反应(real time polymerase chain reaction,RT-PCR)检测肝组织11β-HSD1 mRNA相对表达量,采用Western blot检测肝组织11β-HSD1蛋白相对表达量,于光学显微镜下观察各组大鼠的肝组织病理。结果对照组、模型组和治疗组肝指数分别为(23.4±1.2)mg/g、(25.66±0.99)mg/g、(24.10±1.8)mg/g,差异有统计学意义(F=7.230,P=0.003)。与对照组比较,模型组肝指数显著增高(t=3.725,P<0.001);与模型组比较,治疗组肝指数显著下降(t=2.523,P=0.029)。对照组、模型组和治疗组血液ALT[(42.60±8.78)U/L vs(70.00±11.60)U/L vs(48.90±12.25)U/L]、AST[(99.40±12.99)U/L vs(186.00±20.49)U/L vs(144.7±17.30)U/L]、TC[(1.53±0.17)mmol/L vs(2.11±0.15)mmol/L vs(1.66±0.21)mmol/L]、TG[(0.59±0.06)mmol/L vs(0.68±0.06)mmol/L vs(0.64±0.05)mmol/L]、HDL[(1.05±0.10)mmol/L vs(0.81±0.10)mmol/L vs(0.92±0.09)mmol/L]、FFA[(648±52)μmol/L vs(1081±53)μmol/L vs(764±72)μmol/L]及TNF-α[(0.288±0.068)μg/L vs(0.669±0.051)μg/L vs(0.488±0.054)μg/L]差异均有统计学意义(P均<0.001),与对照组相比,模型组大鼠血液ALT、AST、TC、TG、FFA及TNObjective To investigate the expression of 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1)in rats with non-alcoholic fatty liver disease(NAFLD)and the intervention effect of emodin.Methods Total of 30 male Wistar rats were randomly divided into control group,model group and treatment group,10 cases in each group.Rats in control group were fed with normal diet,and rats in model and treatment group were fed with high-fat diet.The treatment group was given emodin from the 9th week,and the experiment was completed at the end of the 14th week.The hepatic index of rats was calculated,and biochemical indexes of blood and liver tissues were detected,including alaninne aminotransferase(ALT),aspartate aminotransferase(AST),totalcholesterol(TG),triglycerides(TC),high density lipoprotein(HDL),free fatty acid(FFA)and tumor necrosis factorα(TNF-α).The relative expression of 11β-HSD1 mRNA in liver tissue was detected by real time polymerase chain reaction(RT-PCR),and the relative expression of 11β-HSD1 protein was detected by Western blot.Liver histopathology was observed under a light microscope.Results The hepatic indexes of rats in control group,model group and treatment group were(23.4±1.2)mg/g,(25.66±0.99)mg/g and(24.10±1.8)mg/g,respectively,the difference was statistically significant(F=7.230,P=0.003).Compared with control group,hepatic index of rats in model group increased significantly(t=3.725,P<0.001).Compared with model group,hepatic index of rats in the treatment group decreased significantly(t=2.523,P=0.029).The differences of serum ALT[(42.60±8.78)U/L vs(70.00±11.60)U/L vs(48.90±12.25)U/L],AST[(99.40±12.99)U/L vs(186.00±20.49)U/L vs(144.7±17.30)U/L],TC[(1.53±0.17)mmol/L vs(2.11±0.15)mmol/L vs(1.66±0.21)mmol/L],TG[(0.59±0.06)mmol/L vs(0.68±0.06)mmol/L vs(0.64±0.05)mmol/L,HDL[(1.05±0.10)mmol/L vs(0.81±0.10)mmol/L vs(0.92±0.09)mmol/L,FFA[(648±52)μmol/L vs(1081±53)μmol/L vs(764±72)μmol/L]and TNF-α[(0.288±0.068)μg/L vs(0.669±0.051)μg/L vs(0.488±0.054)μg/L]of rats in control group,

关 键 词：11Β-羟基类固醇脱氢酶1 大黄素 脂肪性肝病 非酒精性 大鼠 

分 类 号：R285.5[医药卫生—中药学]