超高效液相色谱-三重四级杆质谱法检测乌鸡白凤丸中鹿角胶特征肽及牛皮源成分  

UPLC-QQQ/MS Analysis of Characteristic Peptide of Deer-horn Glue and Cowhide Source in Wuji Baifeng Pills

作　　者：黄飞燕 李晓君 张国昌 李荣玮 黄晓燕 HUANG Feiyan;LI Xiaojun;ZHANG Guochang;LI Rongwei;HUANG Xiaoyan(West Yunnan University,Lincang Yunnan 677000,China;Comprehensive Technical Center of Mengding Customs,Lincang Yunnan 677000,China;Hunan Institute for Drug Control,Changsha Hunan 410001,China.)

机构地区：[1]滇西科技师范学院,云南临沧677000 [2]孟定海关综合技术中心,云南临沧677000 [3]湖南省药品检验研究院,湖南长沙410001

出　　处：《药品评价》2022年第9期524-528,共5页Drug Evaluation

基　　金：云南省教育厅科学研究基金项目(2021J916);云南省红茶工程技术研究中心项目(2018DH011);湖南省科技厅2020年度自然科学基金科药联合基金项目(2020JJ9002);临沧国家可持续发展议程创新示范区科技专项(202004AC100001-B04)。

摘　　要：目的:建立含胶类中药乌鸡白凤丸中鹿角胶特征肽及可能掺杂的牛皮源成分的专属性检查方法。方法:利用胰蛋白酶对乌鸡白凤丸中所含的胶类特征肽进行酶解,采用超高效液相色谱-三重四级杆质谱,选择鹿角胶特征分子离子峰m/z 765.4(双电荷)→554.0、733.0及牛皮源特征分子离子峰m/z 641.3(双电荷)→726.2、783.3作为检测离子对,ESI+离子化模式,进行多反应监测模式检测。结果:5批乌鸡白凤丸样品均检出了鹿角胶的特征肽段,且均未检出牛皮源成分。结论:该检测方法专属性强、准确、可靠,可用于乌鸡白凤丸中鹿角胶特征肽及牛皮源成分的检测。Objective:To establish an analytical method to identify characteristic peptide of deer-horn glue and cowhide source in wuji baifeng pills.Methods:Identification of characteristic peptide of deer-horn glue and cowhide source in wuji baifeng pills was performed by ultra performance liquid chromatography(UPLC)coupled to mass spectrometry(MS).The characteristic ion pairs of characteristic peptide of deer-horn glue m/z 765.4→554.0,733.0 were as detection ion pairs.The characteristic ion pairs of cowhide source m/z 641.3→726.2,783.3 were as detection ion pairs.The positive electrospray ionization and multiple reaction monitoring were adopted.Results:The characteristic peptide of deer-horn glue was detected out in 5 batches samples,and cowhide source was not detected at the same time.Conclusion:The methods were highly reliable,accurate,and specific,which can be applied to detect the use of deer-horn glue and cowhide in wuji baifeng pills.

关 键 词：乌鸡白凤丸 超高效液相色谱-三重四级杆质谱 鹿角胶 特征肽 牛皮源 

分 类 号：R286.0[医药卫生—中药学]