附子中1个新的苯基异喹啉生物碱及其心肌保护活性  

A novel phenylisoquinoline alkaloid with cardioprotective effect from Aconiti Lateralis Radix Praeparata

作　　者：刘杰 林巧 周勤梅[1,2] 蒙春旺 熊亮 王晓雅[1,2] 缪璐琳 郭力 彭成 LIU Jie;LIN Qiao;ZHOU Qin-mei;MENG Chun-wang;XIONG Liang;WANG Xiao-ya;MIAO Lu-lin;GUO Li;PENG Cheng(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137 [2]成都中医药大学西南特色药材创新药物成分研究所,四川成都611137

出　　处：《中国中药杂志》2022年第12期3265-3269,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重大项目(81891012,81891010);国家自然科学基金青年基金项目(82104546);国家自然科学基金重点项目(81630101);四川省科技计划重点研发项目(2018JZ0081);成都中医药大学“杏林学者”学科人才科研提升计划项目(MPRC2021010)。

摘　　要：采用大孔树脂柱色谱、硅胶柱色谱、制备薄层色谱和半制备高效液相色谱等分离技术从生附片Aconitum carmichaelii酸水提取物中分离得到1个新的9-苯基异喹啉生物碱(1)和1个已知的pavine型异喹啉生物碱(2),并运用多种现代波谱技术对化合物结构进行鉴定,通过计算ECD确定其绝对构型,鉴定为(aS)-7,8-二甲氧基-9-(2-羧基-4,5-二甲氧基苯基)-3,4-二氢异喹啉-1(2H)-酮(1)。采用阿霉素诱导的H9c2大鼠心肌细胞损伤模型评价化合物1和2的心肌细胞保护作用,两者均表现出不同程度的心肌保护活性。Macroporous resin chromatography,silica gel column chromatography,preparative thin layer chromatography,and semi-preparative high performance liquid chromatography were performed to isolate two compounds from the acid extract of the lateral roots of Aconitum carmichaelii:a new 9-phenylisoquinoline alkaloid(1)and a known pavine alkaloid(2).Their structures were elucidated by spectroscopy.The absolute configuration of compound 1 was identified by electronic circular dichroism(ECD)and it was determined to be(aS)-7,8-dimethoxy-9-(2-carboxy-4,5-dimethoxyphenyl)-3,4-dihydroisoquinoline-1(2H)-one(1).The cardioprotective effects of 1 and 2 against doxorubicin-induced toxicity in H9 c2 cells were evaluated.Both of the isoquinoline alkaloids showed cardioprotective activity.

关 键 词：附子 生物碱 异喹啉型 苯基异喹啉 心肌保护 

分 类 号：R284.1[医药卫生—中药学]