基于谱效关系的藁本类药材抗炎活性成分研究  

Anti-inflammatory active component in raw materials of Ligustici Rhizoma et Radix based on spectrum-effect relationship

作　　者：刘久石 张小艺 李斌 魏雪苹 齐耀东[1] 刘海涛[1] 张本刚[1] LIU Jiu-shi;ZHANG Xiao-yi;LI Bin;WEI Xue-ping;QI Yao-dong;LIU Hai-tao;ZHANG Ben-gang(Key Laboratory of Substance Base and Resources Utilization of Chinese Herbal Medicine(Ministry of Education),the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193

出　　处：《中国中药杂志》2022年第12期3295-3302,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81673543);中央本级重大增减支项目(2060302-2002-05)。

摘　　要：研究藁本类药材UPLC指纹图谱与体内抗炎活性的相关性,探究藁本类药材抗炎活性的有效成分群。建立18批藁本类药材的UPLC指纹图谱,测定其中8种化学成分的含量,采用角叉菜胶致小鼠足趾肿胀抗炎模型,测定足肿胀率、IL-1β、IL-6和PGE2,运用典型相关分析法进行谱效关系研究。化学成分聚类分析结果显示藁本和辽藁本的化学成分较为接近。藁本、辽藁本及新疆藁本甲醇提取物均可以显著降低足趾肿胀率,显著下调小鼠肿胀组织中IL-1β、IL-6和PGE2的表达,但新疆藁本的炎症抑制效果弱于藁本和辽藁本。研究结果表明化学成分与药效指标间存在显著的相关关系,UPLC方法中化合物1、3(绿原酸)、4(隐绿原酸)、5、6(阿魏酸)、7(异绿原酸B)、9、11、13、15、16、17、18(阿魏酸松柏酯)、19、20(正丁基苯酞)、21、22、23均与抗炎活性显著相关,其中化合物5、11、13、15、17、21、23与抗炎活性呈负相关。该研究筛选出藁本类药材中抗炎活性的有效成分群,对药材藁本的质量评价体系的完善具有重要意义。The present study investigated the correlation of UPLC fingerprints of raw materials of Ligustici Rhizoma et Radix samples with the anti-inflammatory effect and explored the pharmacodynamic material basis for the anti-inflammatory activity.UPLC fingerprints of 18 batches of raw materials of Ligustici Rhizoma et Radix samples were established for the determination of the content of eight components.The toe swelling rate and the content of IL-1β,IL-6,and PGE2 in rats with toe inflammation induced by carrageenin were measured.Canonical correlation analysis was used to study the spectrum-effect relationship.Cluster analysis indicated that chemical components of Ligusticum sinense and L.jeholense were similar.Methanol extracts of L.sinense,L.jeholense,and Conioselinum vaginatum significantly reduced the toe swelling rate and the content of IL-1β,IL-6 and PGE2 in swollen tissues.The anti-inflammatory effect of C.vaginatum was weaker than that of L.sinense and L.jeholense.The results of spectrum-effect relationship indicated that there was an obvious correlation between chemical components and pharmacodynamic indexes.In UPLC fingerprints,compounds 1,3(chlorogenic acid),4(cryptochlorogenic acid),5,6(ferulic acid),7(isochlorogenic acid B),9,11,13,15,16,17,18(coniferyl ferulate),19,20(N-butylphthalide),21,22,and 23 were significantly correlated with anti-inflammation,among which compounds 5,11,13,15,17,21,and 23 had negative correlation.This study screened out the effective components with anti-inflammatory activity in raw materials of Ligustici Rhizoma et Radix,which was of great significance to improve the quality evaluation system of raw materials of Ligustici Rhizoma et Radix.

关 键 词：藁本类药材 指纹图谱 抗炎活性 谱效关系 

分 类 号：R284.1[医药卫生—中药学]