假单胞菌TCd-1对不同镉耐性水稻品种镉吸收及根际土壤酶活性与镉形态的影响  

Effects of Pseudomonas TCd-1 inoculation on Cd uptake,rhizosphere soils enzyme activities and Cd bioavailability in rice(Oryza sativa)varieties with different Cd tolerance

作　　者：柳玲林 汪敦飞 黄明田 肖清铁 游武[1] 钱鑫 郑新宇 林瑞余 LIU Linglin;WANG Dunfei;HUANG Mingtian;XIAO Qingtie;YOU Wu;QIAN Xin;ZHENG Xinyu;LIN Ruiyu(College of Life Sciences,Fujian Agriculture and Forestry University/Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fuzhou 350002,China;Key Laboratory of Crop Ecology and Molecular Physiology of Fujian Province University,Fuzhou 350002,China)

机构地区：[1]福建农林大学生命科学学院/福建省农业生态过程与安全监控重点实验室,福州350002 [2]福建农林大学作物生态与分子生理学福建省高校重点实验室,福州350002

出　　处：《中国生态农业学报（中英文）》2022年第8期1362-1371,共10页Chinese Journal of Eco-Agriculture

基　　金：国家重点研发计划项目(2017YFD0800900);福建省自然科学基金项目(2009J01056,2013J01083,2015J01081)资助。

摘　　要：为探究假单胞菌TCd-1降低水稻镉吸收的根际生态机制,以高镉耐性水稻品种‘特优671’和低镉耐性水稻品种‘百香139’为材料,通过盆栽土培试验,研究了接种TCd-1菌株对10 mg∙kg^(−1)镉处理水稻镉吸收、根际土壤镉形态及酶活性的影响。结果表明:接种菌株后高、低镉耐性水稻品种各部位的镉含量显著降低(P<0.05),镉富集系数分别降低35.14%和47.79%,转移系数无显著变化;根际土壤可交换态镉含量分别显著降低15.89%和23.81%(P<0.05),铁锰氧化结合态镉含量显著提高39.58%和28.81%(P<0.05),有机态镉含量显著提高36.11%和25.00%(P<0.05);低镉耐性水稻品种根际土壤酸性磷酸酶、脲酶、蔗糖酶、纤维素酶和过氧化氢酶活性依次提高26.74%、12.07%、62.50%、81.17%和5.13%,多酚氧化酶活性降低12.40%,高镉耐性水稻的酸性磷酸酶、脲酶、蔗糖酶、纤维素酶和多酚氧化酶活性依次降低7.19%、9.39%、25.53%、16.20%和11.44%,过氧化氢酶活性提高5.13%。可见,假单胞菌TCd-1主要通过降低根际土壤镉的生物有效性、恢复土壤酶活性,进而提高水稻耐镉能力并抑制水稻对镉的吸收与积累,对高、低不同镉耐性水稻品种,接种TCd-1菌株后其镉富集特性、根际土壤酶活性及不同镉形态含量占比均表现出显著差异。Heavy metal contamination in rice(Oryza sativa)is a serious problem.Microbial remediation is a promising technique to reduce Cd accumulation in rice.To explore the rhizosphere-associated ecological mechanism of Pseudomonas TCd-1-induced reduction of Cd uptake in rice,two rice varieties,high Cd-tolerant variety‘Teyou 671’and low Cd-tolerant variety‘Baixiang 139’,were used.A set of soil culture pots treated with 10 mg∙kg^(−1) Cd were employed to evaluate the effects of Pseudomonas TCd-1 inoculation on rice Cd uptake and enzymes activities in rhizosphere soils.The results showed that the Cd content in different parts of both the high and low Cd-tolerant rice varieties significantly decreased(P<0.05)after inoculation of Pseudomonas TCd-1,and the bioconcentration factor(BCF)of Cd decreased by 35.14%and 47.79%(P<0.05),respectively.However,no significant changes were found in the translocation factor(TF).Meanwhile,in rhizosphere soils of the high and low Cd-tolerant rice varieties,the content of exchangeable Cd decreased by 15.89%and 23.81%(P<0.05),Fe-Mn oxide bound Cd increased by 39.58%and 28.81%(P<0.05),and organic matter Cd increased by 36.11%and 25.00%(P<0.05),respectively.In addition,the activities of acid phosphatase,urease,saccharase,cellulase,and catalase significantly increased by 26.74%,12.07%,62.50%,81.17%,and 5.13%,respectively;while the polyphenol oxidase activity decreased by 12.40%in the rhizosphere soils of low Cd-tolerant rice variety.In rhizosphere soils of high Cd-tolerant rice variety,the activities of acid phosphatase,urease,sucrase,cellulase,and polyphenol oxidase decreased by 7.19%,9.39%,25.53%,16.20%,and 11.44%,respectively;while catalase activity increased by 5.13%.There were significant differences in enrichment characteristics,rhizosphere soil enzymes activities,and the proportions of different chemical forms of Cd in rhizosphere soils of different Cd-tolerant rice varieties after inoculation with the TCd-1 strain.The results indicated that inoculation with the strain may partly r

关 键 词：水稻 镉污染 假单胞菌TCd-1 土壤镉形态 生物有效性 土壤酶活性 微生物修复 

分 类 号：Q939.9[生物学—微生物学] X53[环境科学与工程—环境工程]