HIV感染者外周血滤泡辅助性T细胞活化与血浆IgG水平升高的相关性分析  

Correlation between circulating T follicular helper cells activation and the elevated level of plasma IgG in HIV infected patients

作　　者：陆小凡[1] 李珍[1] 张欣[1] 计云霞[1] 王蕊[1] 吴昊[1] 张彤[1] 粟斌[1] Lu Xiaofan;Li Zhen;Zhang Xin;Ji Yunxia;Wang Rui;Wu Hao;Zhang Tong;Su Bin(Beijing Key Laboratory for HIV/AIDS Research,Clinical and Research Center for Infectious Diseases,Beijing Youan Hospital,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院感染性疾病临床研究中心,艾滋病研究北京市重点实验室,100069

出　　处：《国际病毒学杂志》2022年第3期184-188,共5页International Journal of Virology

基　　金：北京市医管中心人才培养"青苗"、"登峰"计划(QML20181702,DFL20191701);国家自然科学基金项目(82072294,82072271);北京市教育委员会科技计划(km202010025015);艾滋病研究北京市重点实验室(BZ0089)。

摘　　要：目的了解HIV感染者外周血滤泡辅助性T细胞(circulating T follicular helper cell,cTfh)比例与血浆IgG水平的相关性。方法选取未经抗病毒治疗(antiretroviral therapy,ART)的慢性期HIV感染者(chronically HIV infected individuals,CHI),同时筛选HIV抗体阴性的高危暴露者(HIV-exposed seronegative individuals,HESN)作为对照。采用多色流式分析方法,分别检测CHI和HESN的cTfh、ICOS^(+)cTfh、CD40L^(+)cTfh和浆母细胞(plasmablasts,PB)比例。通过免疫散射比浊法检测血浆IgG浓度。结果与HESN相比,CHI组ICOS^(+)cTfh比例(26.97±1.62 vs.18.83±0.99,t=4.518,P<0.01)、PB比例(1.59±0.32 vs.0.46±0.06,t=3.982,P<0.01)和血浆IgG浓度(14.71±0.93 vs.10.65±0.52 g/L,t=4.064,P<0.01)均显著升高。CHI组ICOS^(+)cTfh比例(r=0.586,P=0.030)和PB比例(r=0.372,P=0.038)均与血浆IgG浓度正相关。结论HIV感染导致ICOS^(+)cTfh的增殖可能是引起血浆IgG过度分泌的因素之一,从而引发高丙种球蛋白血症。Objective To understand the correlation between the proportion of circulating T follicular helper cell(cTfh)and the level of plasma IgG in HIV infected patients.Methods Chronically HIV infected subjects(CHI)without antiretroviral therapy(ART)were enrolled and HIV-exposed seronegative individuals(HSEN)were screened as the control.Multicolor flow cytometry was used to analyze the proportions of cTfh,ICOS^(+)cTfh,CD40L^(+)cTfh and plasmablasts(PB)in CHI and HSEN.The level of plasma IgG was determined by turbidimetric inhibition immunoassay.Results Compared to HSEN,the percentage of ICOS^(+)cTfh(26.97±1.62 vs.18.83±0.99,t=4.518,P<0.001),PB(1.59±0.32 vs.0.46±0.06,t=3.982,P<0.001),as well as the level of plasma IgG(14.71±0.93 vs.10.65±0.52 g/L,t=4.064,P<0.001)elevated significantly in CHI.The percentages of ICOS^(+)cTfh(r=0.586,P=0.030)and PB(r=0.372,P=0.038)were positively correlated to the level of plasma IgG in CHI.Conclusions The proliferation of ICOS^(+)cTfh cells caused by HIV infection may be one of the factors related to the over secretion of plasma IgG,and consequently causes hypergammaglobulinemia.

关 键 词：HIV感染 滤泡辅助性T细胞 高丙种球蛋白血症 

分 类 号：R512.91[医药卫生—内科学]