互联网加干血斑HIV核酸检测在MSM人群应用初探  

Application of internet-based dried blood spots testing of HIV nucleic acid among MSM population

作　　者：苏雪丽[1] 郝尹虓 夏冬艳[1] 李砚萍[1] 叶景荣[1] 孙伟东[1] 刘国武[1] 卢红艳[1] Su Xueli;Hao Yinxiao;Xia Dongyan;Li Yanping;Ye Jingrong;Sun Weidong;Liu Guowu;Lu Hongyan(Department of AIDS/STDs Control and Prevention,Beijing Center for Disease Control and Prevention,Beijing 100013,China;Beijing Ren Wei Zhi Ke Technology Co.,Ltd.,Beijing 100021,China)

机构地区：[1]北京市疾病预防控制中心性病艾滋病防治研究所,100013 [2]北京人卫智数科技有限公司,100021

出　　处：《国际病毒学杂志》2022年第3期200-203,共4页International Journal of Virology

基　　金：国家"十三五"科技重大专项(2017ZX10201101);北京市科技重大专项(D171100006717002)。

摘　　要：目的为提高男男性行为(men who have sex with men,MSM)人群艾滋病病毒(human immunodeficiency virus,HIV)发现率,特别是早期感染发现率,探索基于互联网平台和干血斑(dried blood spot,DBS)样本总核酸(total nucleic acid,TNA)检测的新模式在MSM人群中的应用。方法采用滚雪球法招募600名感染状况未知且未经治疗的MSM。DBS的制备和HIV抗体快速检测(rapid test,RT)及TNA检测在北京市疾病预防控制中心进行。若采集到配对静脉血样本,实验室会对其进行抗体酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA);若ELISA筛查阳性,则继续进行HIV抗体免疫印迹(western blot,WB)检测。HIV TNA检测结果提交至互联网平台供被检测人进行查询。结果599份合格样本TNA阳性率为9.35%(56/599),其中RT阴性而TNA阳性百分比为21.4%(12/56)。在RT和TNA均阳性的44个样本中,收集了35份配对的全血样本,其ELISA阳性率为100%(35/35),WB阳性率为91.4%(32/35),WB可疑率为8.57%(3/35)。怀疑早期感染(RT阴性而TNA阳性)的12份样本中,1份配对全血样本ELISA筛查阴性。结论互联网平台加DBS HIV核酸检测可以及时发现高危人群中的感染者,为后续精准干预提供有力支持,对有效防控我国艾滋病疫情具有重要意义。Objective To explore the application of a novel mode of internet-based platform and total nucleic acids(TNA)tests of dried blood spots samples(DBSs)in the population of men who have sex with men(MSM),so as to improve HIV(human immunodeficiency virus)infection discovery,especially early detection of infection.Methods Snowball method was used to recruit 600 untreated MSM with unknown infection status.Preparation of DBSs,as well as antibody rapid tests(RT)and total nucleic acid(TNA)tests of HIV were conducted at the Beijing Center for Disease Prevention and Control.Enzyme linked immunosorbent assay(ELISA)for HIV antibody will be performed in the laboratory for available paired samples of venous blood.In case of ELISA screening positive,western blot(WB)test for HIV antibody will be performed.HIV TNA reports were submitted to internet platform for the inquires by the subjects.Results The positive rate of TNA in 599 qualified samples was 9.35%(56/599)and the percentage of RT negative and TNA positive samples was 21.4%(12/56).Among the 44 samples of RT and TNA positive,35 paired whole blood samples were collected.Positive rate of ELISA was 100%(35/35).The positive rate of WB was 91.4%(32/35),and WB suspicious rate was 8.57%(3/35).Among the 12 samples suspected to be early infections(with RT negative and TNA positive),one paired whole blood sample was ELISA screening negative.Conclusions Internet platform plus DBSs for HIV nucleic acid test detected timely infected patients in high risk population,and provided strong support for subsequent precise interventions.The program is of great significance in effectively preventing and controlling the AIDS epidemics in China.

关 键 词：艾滋病病毒 互联网 干血斑 男男性行为人群 

分 类 号：R512.91[医药卫生—内科学] R440[医药卫生—临床医学]