山东省侧柏枝枯病病原菌鉴定及其致病性测定  

Identification and Pathogenicity of Neofusicoccum occulatum the Agent Shoot Blight of Platycladus orientalis in Shandong Province,China

作　　者：刘在哲 亓玉昆[2] 吕娟 张玉娇 张伟[4] 刘云 付翠翠 刘慇[2] 刘保友[4] 王清海[2] LIU Zai-zhe;QI Yu-kun;LV Juan;ZHANG Yu-jiao;ZHANG Wei;LIU Yun;FU Cui-cui;LIU Yin;LIU Bao-you;WANG Qing-hai(Jinan Forest Farm,Jinan,250014,China;Shandong Provincial Academy of Forestry,Jinan,250014,China;Jinan Academy of Landscape and Forestry,Jinan,250103,China;Yantai Academy of Agricultural Sciences,Yantai 265500,China)

机构地区：[1]济南市林场,山东济南250014 [2]山东省林业科学研究院,山东济南250014 [3]济南市园林和林业科学研究院,山东济南250103 [4]山东省烟台市农业科学研究院,山东烟台265500

出　　处：《林业科学研究》2022年第4期197-204,共8页Forest Research

基　　金：山东省住房和城乡建设厅研究开发项目(2021-K2-6)。

摘　　要：[目的]明确引起山东省济南市侧柏枝枯病的病原菌种类,为该病的防控提供依据。[方法]采用组织分离法和单孢分离法进行病原分离纯化,通过形态学和多基因系统发育分析(ITS、TEF-1α、TUB2)相结合的方法对分离菌株进行鉴定,通过人工接种测定其致病性。[结果]菌落初期白色,菌落中间簇状,后期菌落深灰绿色,背面黑褐色。分生孢子单孢透明,梭形、椭圆形、卵球形。根据形态特征和多基因系统发育分析将分离菌株鉴定为Neofusicoccum occulatum Sakalidis,M.L.,Burgess,T.I.。人工接种分离菌株,均可以引起侧柏枝条、鳞叶发病,症状与田间一致。从发病部位同样可以分离获得接种菌株。[结论]明确了N.occulatum是引起山东省济南市林场侧柏枝枯病的致病菌。[Objective]The pathogens causing shoot blight of Platycladus orientalis(L.)Franco in the Jinan Forest Farm,Shandong province were identified to provide a basis for the prevention and control of the disease.[Method]The pathogens were isolated from the lesion borders of P.orientalis by tissue isolation,and the pure cultures were obtained by monosporic isolation.The isolated strains were identified by combining morphological and polygenic phylogenetic analysis(ITS,TEF-1α,TUB2).[Result]Colonies were initially white,flattened with tufts of white mycelium.After 14 days,mycelium turned to be dark gray-green and dark brown on the back.Conidia are hyaline,unicellular,fusiform,ellipsoid,and cymbiform,ovoid.Three representative isolates(CBL-02,CLB-03,and CBCB-02)were used for pathogenicity tests and phylogenetic analyses.All the isolates tested could cause similar symptoms on the P.orientalis.The multilocus phylogenetic tree was generated using the maximum parsimony method based on 3 genomic loci ITS,TEF-1α,and TUB2.In the maximum likelihood phylogenetic tree,all test isolates were clustered in N.occulatum clade with 89%bootstrap support.Based on the multi-gene phylogeny and morphology,all test isolates were identified as N.occulatum Sakalidis,M.L.,Burgess,T.I.[Conclusion]It is clear that N.occulatum is the pathogenic bacteria causing shoot blight of P.orientalis,and P.orientalis in Jinan City,Shandong Province.

关 键 词：侧柏枝枯病 病原鉴定 形态特征 多基因系统分析 序列 分类 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]