根际细菌LK2-3对黄瓜枯萎病的生物防治作用  

Biocontrol effect of rhizosphere bacteria LK2-3 against Fusarium wilt of cucumber

作　　者：张婉菊 谢太震 陈梦多 王艺茹 沈虎生 何梦菡 朴凤植[2] 申顺善[1] ZHANG Wanju;XIE Taizhen;CHEN Mengduo;WANG Yiru;SHEN Husheng;HE Menghan;PIAO Fengzhi;SHEN Shunshan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,Henan,China;College of Horticulture,Henan Agricultural University,Zhengzhou 450002,Henan,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学园艺学院,郑州450002

出　　处：《中国瓜菜》2022年第7期25-30,共6页China Cucurbits And Vegetables

基　　金：国家现代农业产业技术体系专项(CARS-23-B02)。

摘　　要：LK2-3菌株是从番茄根系分离的植物根际生防细菌。为探讨生防细菌LK2-3对黄瓜枯萎病的生防潜力,采用皿内对峙试验和孢子萌发试验,测定LK2-3对黄瓜枯萎病菌的抑制活性;通过盆栽试验验证其对黄瓜枯萎病的防治效果,并根据形态特征、生理生化特性和16S rRNA序列同源性分析进行鉴定。结果表明,LK2-3菌株对黄瓜枯萎病菌的菌丝生长和孢子萌发均具有显著的抑制作用;在盆栽试验中,LK2-3处理显著促进黄瓜生长,并对黄瓜枯萎病具有明显的防治效果,其防治效果达到88.89%。同时,LK2-3在黄瓜根系和根际土壤具有良好的定殖能力。将LK2-3菌株鉴定为枯草芽孢杆菌(Bacillus subtilis)。综上所述,生防细菌LK2-3防治黄瓜枯萎病具有极大的潜力。A promising biocontrol agent LK2-3 was isolated from roots of tomato.In order to study the biocontrol potential of strain LK2-3 against Fusarium wilt of cucumber,the antifungal activity of LK2-3 against Fusarium oxysporum was detected by in vitro and pot experiments.The results showed that,in vitro experiment,LK2-3 had a significant inhibitory effect on the mycelial growth and germination of spore of F.oxysporum.In the pot experiment,LK2-3 significantly promoted the growth of cucumber and had obvious control effect on Fusarium wilt of cucumber,and its control effect reached 88.89%.Meantime,LK2-3 had a good colonization ability in cucumber root and rhizosphere soil.In addition,based on the morphological,physiological characteristics and 16S rRNA sequence analysis,the strain LK2-3 was identified as Bacillus subtilis.In summary,LK2-3 has good biocontrol potential for Fusarium wilt of cucumber.

关 键 词：黄瓜 枯萎病 生物防治 枯草芽孢杆菌LK2-3 根际定殖 鉴定 

分 类 号：S642.2[农业科学—蔬菜学] S476[农业科学—园艺学]