贝莱斯芽胞杆菌F10促生作用及对烟草青枯病的防治效果  

Tobacco growth promoting effect of Bacillus velezensis F10 and its control effect against tobacco bacterial wilt

作　　者：周向平[1,2] 滕凯 肖启明 陈武[1] 王凯歌[2] 张洪波 刘峰 刘天波 ZHOU Xiangping;TENG Kai;XIAO Qiming;CHEN Wu;WANG Kaige;ZHANG Hongbo;LIU Feng;LIU Tianbo*(College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;Yongzhou Branch of Hunan Provincial Tobacco Company,Yongzhou 425000,Hunan,China;Xiangxi Branch of Hunan Provincial Tobacco Company,Jishou 416000,Hunan,China;Hunan Research Institute of Tobacco Science,Changsha 410004,China)

机构地区：[1]湖南农业大学植物保护学院,长沙410128 [2]湖南省烟草公司永州市公司,湖南永州425000 [3]湖南省烟草公司湘西自治州公司,湖南吉首416000 [4]湖南省烟草科学研究所,长沙410004

出　　处：《烟草科技》2022年第7期9-16,共8页Tobacco Science & Technology

基　　金：湖南省自然科学基金项目“短短芽胞杆菌B011抗真菌天然产物的纯化鉴定及其编码基因/簇的克隆”(2019JJ40119);湖南省重点研发计划项目“湖南省优势种植业功能微生物种质资源挖掘、选育与创制”(2019NK2192);中国烟草总公司湖南省公司科技项目“防治烟草主要根茎病害的生物型菌肥与应用”(17-20Aa01)。

摘　　要：为明确植物根际促生细菌(Plant growth-promoting rhizobacteria,PGPR)对烤烟促生和抗病作用,以前期试验分离获得的贝莱斯芽胞杆菌(Bacillus velezensis)F10为材料,通过盆栽和田间小区试验考察其对烟草的促生作用和对烟草青枯病的防治效果。结果表明,生防菌F10处理后,烟草根系和地上部生长显著优于对照,烟草叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性提高。盆栽和田间小区试验中生防菌F10对烟草青枯病的防治效果分别为60.49%和54.91%,大田烟叶产量、上等烟比例和上中等烟比例比对照分别提高11.82%、31.46%和13.17%。生防菌F10对烟草生长具有促进作用,对烟草青枯病具有较好的防治效果,烤烟经济效益显著提高。To clarify the effects of plant growth promoting rhizobacteria(PGPR)on growth promotion and disease resistance of flue-cured tobacco,potential roles of previously isolated Bacillus velezensis F10 were studied through pot and plot experiments to investigate its growth promotion and control effects on tobacco bacterial wilt.The results showed that the growth of roots and aerial parts of tobacco plants under the biocontrol bacterium F10 treatment were significantly better than that of the control,and the activities of peroxidase(POD),superoxide dismutase(SOD)and catalase(CAT)in tobacco leaves increased.The control effects of biocontrol bacterium F10 on tobacco bacterial wilt in pot and plot experiments were 60.49%and 54.91%respectively.The agronomic traits of flue-cured tobacco in fields treated with biocontrol bacterium F10 were significantly better than those of the control.The yield,output value,average price,proportion of high grade leaves,and proportion of high-to-mid grade leaves increased by 11.82%,31.46%and 13.17%respectively.Biocontrol bacterium F10 has growth promoting effect on flue-cured tobacco and has good control effect against tobacco bacterial wilt,which significantly promotes the economic benefits of flue-cured tobacco.

关 键 词：烟草 根际促生细菌 贝莱斯芽胞杆菌 烟草青枯病 促生作用 防治效果 

分 类 号：S572.01[农业科学—烟草工业]