Integrative taxonomy helps to reveal the mask of the genus Gynandropaa (Amphibia: Anura: Dicroglossidae)  

作　　者：Yan HUANG Junhua HU Bin WANG Zhaobin SONG Caiquan ZHOU Jianping JIANG 

机构地区：[1]Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,China [2]College of Life Science,Sichuan University,Chengdu,China [3]University of Chinese Academy of Sciences,Beijing,China [4]Key Laboratory of Southwest China Wildlife Resources Conservation,China West Normal University,Ministry of Education,Nanchong,China

出　　处：《Integrative Zoology》2016年第2期134-150,共17页整合动物学（英文版）

基　　金：support­ed by the National Natural Science Foundation of China(31471964,31071906;31270568);the Youth Innovation Promotion Association CAS(2015304),the Sichuan Province Distinguished Youth Fund(2014JQ0056);the West Light Foundation CAS(Y4C3031100).

摘　　要：Species of the genus Gynandropaa within the family Dicroglossidae are typical spiny frogs whose taxonomic status has long been in doubt.We used integrative methods,involving morphological and molecular analyses,to elucidate the phylogenetic relationships,and to determine identities and the geographic distribution of each val­id species.We obtained DNA sequence data of 5 species of Gynandropaa(complete sequences of the mitochon­drial NADH dehydrogenase subunit 2[ND2]gene,and 890 bp of 12S rRNA and 16S rRNA partial sequences)from 37 localities(including the topotypes of 5 described species)and constructed Bayesian and maximum-like­lihood trees to examine the patterns of phylogeography.A total of 28 morphological variables were taken on 624 specimens.Three clades with clear geographic patterns were recognized:clade C(from south-western Sich­uan Province and central Yunnan Province),clade E(western Guizhou Province and eastern to central Yunnan Province)and clade W(western to southern Yunnan Province).Integrating morphological characteristics and distribution information,the clades W,E and C represent Gynandropaa yunnanensis,G.phrynoides and G.si­chuanensis,respectively.We draw the following conclusions:(i)the taxon G.phrynoides,formerly evaluated as a junior synonym of G.yunnanensis,is revalidated herein at the rank of species;(ii)G.liui is a junior synonym of G.sichuanensis;and(iii)G.yunnanensis is a valid species while G.bourreti is probably a subspecies of G.yunnanensis,with the distribution range from Vietnam to southern Yunnan Province.This study clears up the taxonomic status of Gynandropaa and provides important information for understanding the evolution and con­servation of these spiny frogs.

关 键 词：AMPHIBIANS morphological traits spiny frogs mitochondrial DNA SYSTEMATICS 

分 类 号：F42[经济管理—产业经济]