Exploring novel candidate genes from the Mouse Genome Informatics database: Potential implications for avian migration research  

作　　者：Andrea CONTINA Eli S.BRIDGE Jeffrey F.KELLY 

机构地区：[1]Oklahoma Biological Survey,University of Oklahoma,Norman,Oklahoma,USA [2]Department of Biology,University of Oklahoma,Norman,Oklahoma,USA

出　　处：《Integrative Zoology》2016年第4期240-249,共10页整合动物学（英文版）

基　　金：Funding for this research came from the Oklahoma Biological Survey,the Na­tional Science Foundation(IDBR#1152356,and DEB#0946685);the United States Department of Agri­culture-National Institute of Food and Agriculture(NI­FA-AFRI-003536);All work with animals was per­formed under state and federal banding permits(Permit#23215);was approved by the Institu­tional Animal Care and Use Committee of the Univer­sity of Oklahoma(Protocol#R12-019).

摘　　要：To search for genes associated with migratory phenotypes in songbirds,we selected candidate genes through annotations from the Mouse Genome Informatics database and assembled an extensive candidate-gene library.Then,we implemented a next-generation sequencing approach to obtain DNA sequences from the Painted Bun­ting genome.We focused on those sequences that were conserved across avian species and that aligned with candidate genes in our mouse library.We genotyped short sequence repeats from the following candidate genes:ADRA1d,ANKRD17,CISH and MYH7.We studied the possible correlations between allelic variations occur­ring in these novel candidate migration genes and avian migratory phenotypes available from the published lit­erature.We found that allele variation at MYH7 correlated with a calculated index of speed of migration(km/day)across 11 species of songbirds.We highlight the potential of the Mouse Genome Informatics database in providing new candidate genes that might play a crucial role in regulating migration in birds and possibly in other taxa.Our research effort shows the benefits and limitations of working with extensive genomic datasets and offers a snapshot of the challenges related to cross-species validation in behavioral and molecular ecology studies.

关 键 词：avian migration candidate gene approach Mouse Genome Informatics MYH7 SONGBIRDS 

分 类 号：R71[医药卫生—妇产科学]