Isolation of microsatellite loci and reliable genotyping using noninvasive samples of a critically endangered primate, Trachypithecus leucocephalus  

作　　者：Weiran WANG Yu QIAO Yitao ZHENG Meng YAO 

机构地区：[1]School of Life Sciences,Peking University,Beijing,China

出　　处：《Integrative Zoology》2016年第4期250-262,共13页整合动物学（英文版）

基　　金：supported by the Natural Science Foundation of China(Grant No.31272329);Project 985 Research Fund to M.Y.

摘　　要：Genetic information can be critical in identifying conservation priorities and developing conservation strategies.There is an urgent need for noninvasive genetic tools to study the wild populations of Asian colobine monkeys.The majority of these species are threatened with habitat destruction,population reduction and even extinction,but generally lack information on their genetic diversity and population structure.Genetic sampling and tissue collection have been scarce in these species owing to strict regulations on manipulation of endangered species,and the difficulties and risks associated with capturing these arboreal and fast-moving monkeys in the challeng­ing environments that they inhabit.These difficulties have hindered the development of molecular genetic mark­ers,which are usually derived from tissues or blood.In this study,we present a method for de novo microsatel­lite isolation and genotyping using DNA from noninvasive origins of a critically endangered Asian colobine,the white-headed langur(Trachypithecus leucocephalus).Genomic DNA isolated from hair was shown to be suf­ficient for microsatellite enrichment and isolation,with similar isolation efficiencies as from tissue DNA.We identified and characterized 20 polymorphic microsatellite loci,and evaluated their amplification success and genotyping reliability with 86 field-collected fecal samples.These results show that this panel of loci can pro­duce reliable genotypes from fecal samples,and represent a useful tool for noninvasive investigation of genetic structure,individual identification and kinship assessment in this highly endangered species.Our approach can be applied to conservation genetic studies of other wild species that lack sequence information and tissue sam­ples.

关 键 词：Asian colobine conservation genetics FIASCO HAIR scatology 

分 类 号：Q94[生物学—植物学]