Unrecognized diversity in New Guinean crayfish species (Decapoda, Parastacidae): The evidence from molecular data  

作　　者：Martin BLÁHA Jiří PATOKA Pavel KOZÁK Antonín KOUBA 

机构地区：[1]Faculty of Fisheries and Protection of Waters,South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses,University of South Bohemia inČeskéBudějovice,Vodňany,Czech Republic [2]Department of Zoology and Fisheries,Faculty of Agrobiology,Food and Natural Resources,Czech University of Life Sciences Prague,Praha,Czech Republic

出　　处：《Integrative Zoology》2016年第6期457-468,共12页整合动物学（英文版）

基　　金：supported by CIGA(project no.20152007);the Ministry of Edu­cation,Youth and Sports of the Czech Republic(projects“CENAKVA”[no.CZ.1.05/2.1.00/01.0024];“CENAK­VA II”[no.LO1205 under the NPU I program]and“The Development of Postdoc Positions at the USB”[no.CZ.1.07/2.3.00/30.0049];Partial funding was pro­vided by the Czech Science Foundation through project P502/12/P177.

摘　　要：The phylogenetic relationships among imported ornamental crayfish belonging to the genus Cherax were in­ferred from a combined dataset of 3 mitochondrial genes(COI,16S and 12S)and by comparison with avail­able GenBank sequences of 14 Cherax species.Furthermore,the concordance of previously described species obtained from a wholesaler(Cherax boesemani,C.holthuisi and C.peknyi)with available GenBank sequences was verified based on COI with special respect to comparison with sequences assigned as Cherax species.Re­cently described species C.gherardiae,C.pulcher and C.subterigneus belong to the northern group of Cher­ax species.Comparison and analysis with other GenBank COI sequences show previously unreported diversity of New Guinean species,suggesting 5 putative new species.Surprisingly,species assigned to the subgenus As­taconephrops do not form a monophyletic clade;this subgenus should be reappraised relative to the purported typical morphological characteristic of the uncalcified patch on male chelae.Increasing importation of crayfish underscores the importance of accurate species identification.Use of basic molecular methods is a necessary requisite for documenting occurrence,abundance and population trends of target species.Consequently,it helps to support eventual conservation decision-making by stakeholders.

关 键 词：CHERAX cytochrome c oxidase DNA barcoding mitochondrial DNA ornamental crayfish 

分 类 号：Q95[生物学—动物学]