5-氨基乙酰丙酸对吴屯杨幼苗盐碱胁迫的影响  

Mitigation Effect of Exogenous 5-aminolevulinic Acid on Populus wutunensis Seedling under Saline-alkali Stress

作　　者：刘欢 王铸 董爽 邹吉祥 金华 Liu Huan;Wang Zhu;Dong Shuang;Zou Jixiang;Jin Hua(Dalian Minzu University,Dalian 116600,P.R.China;Changji National High-Tech Industrial Development Zone Administration Committee;Dalian Minzu University)

机构地区：[1]大连民族大学环境与资源学院,大连116600 [2]新疆昌吉高新技术产业开发区管理委员会 [3]大连民族大学

出　　处：《东北林业大学学报》2022年第7期47-52,共6页Journal of Northeast Forestry University

基　　金：中央财政林业科技推广示范资金项目(辽[2019]TG01号)。

摘　　要：为探讨外源5-氨基乙酰丙酸(ALA)缓解植物盐碱胁迫伤害的生理机制,以1年生吴屯杨幼苗为试验材料,通过浓度为50 mg·L^(-1)5-氨基乙酰丙酸的处理,分析5-氨基乙酰丙酸对盐碱(200 mmol·L^(-1)的M(NaCl)∶M(NaHCO_(3))=1∶1溶液)胁迫下的吴屯杨幼苗抗氧化酶活性、渗透调节物质的量、叶片细胞膜透性、光合色素质量分数、光合参数、气孔特征以及蜡质结构的影响。结果表明:与对照相比,盐碱胁迫下吴屯杨幼苗的丙二醛质量摩尔浓度和相对电导率分别增加了32.71%和88.43%,光合速率降低,蜡质破碎,植物生长受到抑制;喷施5-氨基乙酰丙酸并盐碱胁迫的植株比仅进行盐碱胁迫的植株丙二醛质量摩尔浓度显著下降了32.97%,光合速率升高,超氧化物歧化酶、过氧化物酶和过氧化氢酶等抗氧化酶活性分别升高了34.60%、25.45%和20.57%,蜡质结构的破碎情况得到了缓解。喷施5-氨基乙酰丙酸可有效提高吴屯杨叶片光合特性和抗氧化能力,缓解活性氧伤害,从而增强吴屯杨幼苗对盐碱胁迫的适应能力。The purpose of this study was to explore the physiological mechanism of exogenous 5-aminolevulinic acid(ALA)alleviating the damage caused by saline-alkali stress in plants.One-year-old seedlings of Populus wutunensis were subjected to saline-alkali stress(200 mmol·L^(-1)saline-alkali solution molar ratio of NaCl∶NaHCO_(3)is 1∶1),and then the seedlings were treated with 50 mg·L^(-1)ALA.The activity of antioxidant enzymes,contents osmotic adjustment substances,leaf cell membrane permeability,chlorophyll content,photosynthetic parameters,stomatal characteristics and wax in P.wutunensis seedlings were analyzed.Compared with the control,the MDA content and relative conductivity of P.wutunensis seedlings increased by 32.71%and 88.43%,respectively,the photosynthetic rate decreased,the wax was broken,and the plant growth was inhibited.Compared with the saline-alkali stress only,the application of ALA significantly reduced the malondialdehyde content by 32.97%,increased the photosynthetic rate,increased the activities of antioxidant enzymes such as SOD,POD and CAT by 34.60%,25.45%and 20.57%,respectively,and alleviated the breakage of wax.In conclusion,the application of 50 mg·L^(-1)ALA can effectively improve the photosynthetic characteristics and antioxidant capacity of P.wutunensis leaves,and reduce the damage of reactive oxygen species,thus enhancing the adaptability of P.wutunensis seedlings to saline-alkali stress.

关 键 词：5-氨基乙酰丙酸 盐碱胁迫 吴屯杨 生理响应 

分 类 号：S715.3[农业科学—林学]