MBG/PGA-PCL composite scaffolds provide highly tunable degradation and osteogenic features  

作　　者：Jiangfeng Li Chunyi Wang Guoxing Gao Xing Yin Ximing Pu Bing Shi Yang Liu Zhongbing Huang Juan Wang Jingtao Li Guangfu Yin 

机构地区：[1]College of Biomedical Engineering,Sichuan University,Chengdu,610065,PR China [2]State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,West China Hospital of Stomatology,Sichuan University,Chengdu,610041,PR China [3]State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,Department of Orthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu,610041,PR China [4]Shandong Provincial Key Laboratory of Biomedical Polymers,Shandong Academy of Pharmaceutical Sciences,Jinan,250101,PR China

出　　处：《Bioactive Materials》2022年第9期53-67,共15页生物活性材料（英文）

基　　金：supported by National Key R&D Program of China(2018YFC1105703);Key R&D Program of Sichuan Province(2017SZ0047).

摘　　要：It remains a challenge to achieve satisfactory balance between biodegradability and osteogenic capacity in biosynthetic bone grafts.In this study,we aimed to address this challenge by incorporating mesoporous bioactive glass(MBG)into poly(caprolactone-co-glycolide)(PGA-PCL)at gradient ratios.MBG/PGA-PCL(PGC/M)scaffolds with MBG incorporation ratio at 0,10%,25%and 40%(PGC/M0-40)were synthesized using a modified solvent casting-particulate leaching method,and their physiochemical and biological properties were comprehensively evaluated.PGC/M scaffolds exhibited highly perforated porous structure with a large-pore size of 300-450μm,with ordered MBGs of around 6.0 nm mesopores size uniformly dispersed.The increase in MBG incorporation ratio significantly improved the scaffold surface hydrophilicity,apatite-formation ability and pH stability,increased the weight loss rate while insignificantly influenced the molecular chains degradation of PGA-PCL component,and facilitated the attachment,spreading,viability and proliferation of rat bone marrow stromal cells(rBMSCs)on scaffolds.Moreover,rBMSCs cultured on PGC/M10-40 scaffolds demonstrated enhanced ALP activity and osteogenesis-related gene expression in a MBG dose-dependent manner as compared with those cultured on PGC/M0 scaffolds.When implanted to the rat cranial bone defect,PGC/M25 and PGC/M40 scaffolds induced significantly better bone repair as compared to PGC/M0 and PGC/M10 scaffolds.Besides,the biodegradability of PGC/M scaffolds correlated with the MBG incorporation ratio.These data suggested this novel PGC/M scaffolds as promising bone repair biomaterial with highly tunable hydrophilicity,bioactivity,cytocompatibility,osteogenic activity as well as biodegradability.

关 键 词：Mesoporous bioactive glasses Poly(caprolactone-co-glycolide) Osteogenic differentiation Bone repair 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]