Eco-friendly bacteria-killing by nanorods through mechano-puncture with top selectivity  

作　　者：Jing Ye Bo Li Yufeng Zheng Shuilin Wu Dafu Chen Yong Han 

机构地区：[1]State Key Laboratory for Mechanical Behavior of Materials,Xi’an Jiaotong University,Xi’an,710049,China [2]Center for Biomedical Materials and Tissue Engineering,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,100871,China [3]School of Materials Science&Engineering,The Key Laboratory of Advanced Ceramics and Machining Technology by the Ministry of Education of China,Tianjin University,Tianjin,300072,China [4]Laboratory of Bone Tissue Engineering,Beijing Research Institute of Traumatology and Orthopaedics,Beijing Jishuitan Hospital,Beijing,100035,China

出　　处：《Bioactive Materials》2022年第9期173-184,共12页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(Grant number 51631007,51971171 and 3700860);the joint project of Xi’an Jiaotong University and Beijing Research Institute of Traumatology and Orthopaedics(Contract No.202012443)for financially supporting this work.

摘　　要：Nanorods can induce mechano-puncture of Staphylococcus aureus(S.aureus)that often impairs osseointegration of orthopedic implants,while the critical nanorod top sharpness able to puncture S.aureus and the predominant contributor between top sharpness and length to mechano-puncture activity remains elusive.Herein,we fabricated three kinds of Al2O3-wrapped nanorods patterned arrays with different lengths and top sharpness.The top-sharp nanorods have lengths of 469 and 884 nm and the shorter show a length identical to the top-flat nanorods.Driven by the equivalent adhesive force of S.aureus,the top-flat nanorods deform cell envelops,showing a bacteriostatic rate of 29%owing to proliferation-inhibited manner.The top-sharp nanorods puncture S.aureus,showing a bactericidal rate of 96%for the longer,and 98%for the shorter that simultaneously exhibits fair osseointegration in bacteria-infected rat tibias,identifying top sharpness as a predominate contributor to mechano-puncture activity.Based on finite-element simulation,such top-flat nanorod derives the maximum stress(Smax)of 5.65 MPa on cell wall,lower than its ultimate-tensile-strength(13 MPa);while such top-sharp and shorter nanorod derives Smax of 20.15 MPa to puncture cell envelop.Moreover,a critical top conical angle of 138◦is identified for nanorods able to puncture S.aureus.

关 键 词：ANTI-BACTERIA NANORODS Top sharpness Mechano-puncture Finite element simulation 

分 类 号：R318[医药卫生—生物医学工程]