猪支气管败血波氏杆菌主要毒力基因的检测  

Detection of Main Virulence Genes in Bordetella bronchiseptica Strains from Swine

作　　者：张瑞华 于永乐 张鸿 李焕发 高善颂[1] 陈超[1] 张丹萍 窦心怡 韩先杰[1] ZHANG Rui-hua;YU Yong-le;ZHANG Hong;LI Huan-fa;GAO Shan-song;CHEN Chao;ZHANG Dan-ping;DOU Xin-yi;HAN Xian-jie(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学动物医学院,山东青岛266109

出　　处：《中国兽医杂志》2022年第5期29-32,38,共5页Chinese Journal of Veterinary Medicine

基　　金：山东省重大科技创新工程项目(2019JZZY010720)。

摘　　要：为了解山东省胶东地区猪支气管败血波氏杆菌主要毒力基因的分布,本试验通过革兰染色、生化试验和PCR等方法对分离到的8株细菌进行鉴定;利用PCR方法对分离株的主要毒力基因进行检测。结果显示,8株分离菌均为猪支气管败血波氏杆菌,均含有菌毛基因、外源铁载体受体基因、腺苷酸环化酶溶血素基因、内膜感受器组氨酸激酶基因和百日咳杆菌黏附素基因,此外7株分离菌还包含菌毛血凝素基因和皮肤坏死毒素基因。结果表明,山东省胶东地区猪支气管败血波氏杆菌分离株均拥有毒力基因,其有效防控存在挑战。In order to determine the presence of major virulence genes in swine Bordetella bronchiseptica from Jiaodong area of Shandong province,8 bacterial strains of swine origins were identified by Gram staining,biochemical test and PCR,and their possession of major virulence genes was verified by PCR.The results showed that all 8 strains of swine origins were B.bronchiseptica,and all strains contained the fimbriae,bfrZ,adenylate cyclase-hemolysin,bvgs,and pertactin genes,and 7 strains also contained the filamentous hemagglutinin and dermonecrotic toxin genes.Thus,B.bronchiseptica strains from swine in Jiaodong area of Shandong province possess major virulence genes,which create challenges for their effective control and eradication.

关 键 词：猪 支气管败血波氏杆菌 毒力基因 PCR 

分 类 号：S858.92[农业科学—临床兽医学]