Microcluster colloidosomes for hemostat delivery into complex wounds:A platform inspired by the attack action of torpedoes  

作　　者：Bitao Lu Enling Hu Ruiqi Xie Kun Yu Fei Lu Rong Bao Chenhui Wang Guangqian Lan Fangyin Dai 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing,400715,China [2]Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile,Chongqing,400715,China [3]The Ninth People’s Hospital of Chongqing No.69 Jialing Village,BeiBei District,Chongqing,400715,China [4]Chongqing Key Laboratory of Natural Product Synthesis and Drug Research,School of Pharmaceutical Sciences,Chongqing University,55 South Daxuecheng Road,Chongqing,401331,China

出　　处：《Bioactive Materials》2022年第10期372-387,共16页生物活性材料（英文）

基　　金：supported by the China Agriculture Research System(No.CARS-18-ZJ0102);National Natural Science Foundation of China(Nos.52103096,51803170,51803171,and 81703424);Natural Science Foundation of Chongqing,China(grant number cstc2020jcyj-msxmX0383);Fundamental Research Funds for the Central Universities(2020CDJQY-A041).

摘　　要：Complex yet lethal wounds with uncontrollable bleeding hinder conventional hemostats from clotting blood at the source or deep sites of injury vasculature,thereby causing massive blood loss and significantly increased mortality.Inspired by the attack action of torpedoes,we synthesized microcluster(MC)colloidosomes equipped with magnetic-mediated navigation and“blast”systems to deliver hemostats into the cavity of vase-type wounds.CaCO_(3)/Fe_(2)O_(3)(CF)microparticles functionalized with Arg-Gly-Asp(RGD)modified polyelectrolyte multilayers were co-assembled with oppositely charged zwitterionic carbon dots(CDs)to form MC colloidosomes,which were loaded with thrombin and protonated tranexamic acid(TXA-NH_(3)^(+)).The composite microparticles moved against blood flow under magnetic mediation and simultaneously disassembled for the burst release of thrombin stimulated by TXA-NH_(3)^(+).The CO_(2) bubbles generated during disassembly produced a“blast”that propelled thrombin into the wound cavity.Severe bleeding in a vase-type hemorrhage model in the rabbit liver was rapidly controlled within~60 s.Furthermore,in vivo subcutaneous muscle and liver implantation models demonstrated excellent biodegradability of MC colloidosomes.This study is the first to propose a novel strategy based on the principle of torpedoes for transporting hemostats into vase-type wounds to achieve rapid hemostasis,creating a new paradigm for combating trauma treatment.

关 键 词：Torpedoes-inspired Microcluster colloidosomes Magnetic guidance DISINTEGRATION HEMOSTASIS 

分 类 号：R318.08[医药卫生—生物医学工程]