Osteogenic lithium-doped brushite cements for bone regeneration  

作　　者：K.Hurle F.R.Maia V.P.Ribeiro S.Pina J.M.Oliveira F.Goetz-Neunhoeffer R.L.Reis 

机构地区：[1]GeoZentrum Nordbayern,Mineralogy,Friedrich-Alexander University Erlangen-Nürnberg(FAU),91054,Erlangen,Germany [2]B’s Research Group,I3Bs–Research Institute on Biomaterials,Biodegradables and Biomimetics,University of Minho,Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine,AvePark,Parque de Ciˆencia e Tecnologia,Zona Industrial da Gandra,4805-017,Barco,Guimar˜aes,Portugal [3]ICVS/3B’s–PT Government Associate Laboratory,Braga,Guimar˜aes,Portugal

出　　处：《Bioactive Materials》2022年第10期403-417,共15页生物活性材料（英文）

基　　金：funded by the Portuguese Foundation for Science and Technology(FCT)and the German Academic Exchange Service(Deutscher Akademischer Austauschdienst,DAAD)for the transnational cooperation FCT/DAAD 2018-2019;FRM acknowledges her contract under the Transitional Rule DL 57/2016(CTTI-57/18-I3BS(5))attributed by the FCT.VPR acknowledges the Junior Researcher contracts(POCI-01-0145-FEDER-031367;POCI-01-0145-FEDER-029139)under the projects Fun4TE project(PTDC/EMD-EMD/31367/2017)and B-Liver(PTDC/EMD-EMD/29139/2017)attributed by the FCT.The authors also thank the funds provided under the distinctions attributed to JMO(IF/01285/2015)and SP(CEECIND/03673/2017).Furthermore,funding by the German Research Foundation(Deutsche Forschungsgemeinschaft,DFG),Grant Nr.HU 2498/1-1;GB 1/22-1,is acknowledged.

摘　　要：This study investigated the osteogenic performance of new brushite cements obtained from Li+-dopedβ-tricalcium phosphate as a promising strategy for bone regeneration.Lithium(Li+)is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells(hASCs)and the osteogenic differentiation-related gene expression,essential for osteogenesis.In-situ X-ray diffraction(XRD)and in-situ 1H nuclear magnetic resonance(1H NMR)measurements proved the precipitation of brushite,as main phase,and monetite,indicating that Li+favored the formation of monetite under certain conditions.Li+was detected in the remaining pore solution in significant amounts after the completion of hydration.Isothermal calorimetry results showed an accelerating effect of Li+,especially for low concentration of the setting retarder(phytic acid).A decrease of initial and final setting times with increasing amount of Li+was detected and setting times could be well adjusted by varying the setting retarder concentration.The cements presented compressive mechanical strength within the ranges reported for cancellous bone.In vitro assays using hASCs showed normal metabolic and proliferative levels.The immunodetection and gene expression profile of osteogenic-related markers highlight the incorporation of Li+for increasing the in vivo bone density.The osteogenic potential of Li-doped brushite cements may be recommended for further research on bone defect repair strategies.

关 键 词：Lithium doping β-tricalcium phosphate Brushite cements Alkaline phosphatase activity Collagen expression Osteogenic expression Bone regeneration 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]