机构地区:[1]Shanghai Key Laboratory of Orthopedic Implant,Department of Orthopedic Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,No.639 Zhizaoju Rd,Shanghai,200011,China [2]Department of Orthopaedic Surgery,Children’s Hospital of Nanjing Medical University,No.72 Guangzhou Rd,Nanjing,210008,China [3]Department of Rehabilitation,Huashan Hospital,Fudan University,No.12 Middle Urumqi Rd,Shanghai,200040,China [4]Med-X Research Institute,School of Biomedical Engineering,Shanghai Jiao Tong University,No.1956 Huashan Rd,Shanghai,200030,China [5]Department of Orthopedics,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,No.85 Wujin Rd,Shanghai,200080,China
出 处:《Bioactive Materials》2022年第11期162-177,共16页生物活性材料(英文)
基 金:The study was supported by the National Key R&D Program of China(2018YFA0703000);China Postdoctoral Science Foundation(2020M681320);National Nature Science Foundation of China(grant nos.82072969);Jiangsu Provincial Key Research and Development Program(CN)(BE2019608);National Natural Science Foundation of China(82072412/81772326);Project of Shanghai Science and Technology Commission(19XD1434200/18431903700).
摘 要:Uncontrolled hemorrhage and wound infection are crucial causes of trauma-associated death in both the military and the clinic. Therefore, developing an efficient and rapid hemostatic method with biocompatibility, easy degradation, and wound healing is of great importance and desirability. Inspired by spontaneous blood cell plug formation in the hemostasis process, an adenosine 5′-diphosphate modified pro-coagulation hyaluronic acid (HA-ADP) coordinated with enhanced antibacterial activity of undecanal-modified chitosan (UCS) was fabricated through physical electrostatic cross-linking and freeze-drying. The as-prepared hydrogel sponges showed a porous structure suitable for blood cell adhesion. In particular, the hydrogel exhibited excellent antibacterial ability and promoted the adhesion of platelets and red blood cells, thus inducing a prominent pro-coagulation ability via platelet activation, which exhibits a shorter hemostasis time (58.94% of control) in vitro. Compared with commercially available CELOX and gelatin sponge (GS), HA-ADP/UCS accelerates hemostasis and reduces blood loss in both rat tail amputation and rat artery injury models. Furthermore, all the samples exhibited su-perior cytocompatibility and biodegradability. Due to these performances, HA-ADP/UCS promoted full-thickness skin defect healing significantly in vivo. All the properties of HA-ADP/UCS suggest that it has great potential for translation as a clinical application material for hemostatic and wound healing.
关 键 词:HEMOSTASIS Antibacterial Wound healing Hyaluronic acid Chitosan
分 类 号:R318[医药卫生—生物医学工程]
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