1株南美蟛蜞菊内生真菌Aspergillus sp.PQJ-1次级代谢产物及其抗菌活性研究  

Studies on Secondary Metabolites from Sphagneticola trilobata Fungus Aspergillus sp. PQJ-1 and Their Antibacterial Activities

作　　者：李文杏 王一诺 马涛 张荣浚 惠阳[1,2] 陈文豪[1,2] LI Wenxing;WANG Yinuo;MA Tao;ZHANG Rongjun;HUI Yang;CHEN Wenhao(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education/College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;Key Laboratory of Tropical Medicinal Plant Chemnistry of Hainan Province/College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China)

机构地区：[1]热带药用资源化学教育部重点实验室/海南师范大学化学与化工学院,海南海口571158 [2]热带药用植物化学海南省重点实验室/海南师范大学化学与化工学院,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2022年第2期141-145,共5页Journal of Hainan Normal University（Natural Science）

基　　金：海南省重点研发项目(ZDYF2021SHFZ221);海南省自然科学基金(221RC541);海南省高等学校科学研究项目(Hnky2021ZD-10);大学生创新训练项目(S202111658008)。

摘　　要：采用硅胶、Sephadex LH-20、半制备HPLC等柱色谱方法,对南美蟛蜞菊内生真菌Aspergillus sp. PQJ-1发酵产物的乙酸乙酯提取物进行分离纯化,得到6个单体化合物。利用NMR、MS等波谱方法以及与参考文献对照,鉴定它们的结构分别为:cyclo-D-Trp-L-Pro(1),1H-indole-3-carboxaldehyde(2),isoeugenin(3),flufaran(4),aflatoxinB1(5),sterigmatocystin(6)。对所有化合物进行抗菌活性测试,结果表明,化合物1、2、4~6对耐甲氧西林金黄色葡萄球菌、白色念珠菌显示出抗菌活性。Six compounds were isolated from the EtOAc extract of the fermentation product of the fungus Aspergillus sp.PQJ-1 from Sphagneticola trilobata using silica gel, Sephadex LH-20, and semi-preparative HPLC column chromatography methods. Their structures were identified as cyclo-D-Trp-L-Pro(1), 1H-indole-3-carboxaldehyde(2), isoeugenin(3), flufaran(4), aflatoxinB1(5), sterigmatocystin(6) by using NMR、MS spectroscopic analysis and comparison with the references. All compounds were tested for their antibacterial activities, and the results showed that compounds 1, 2, 4~6 exhibited antibacterial activities against methicillin-resistant Staphylococcus aureus and Candida albicans.

关 键 词：南美蟛蜞菊 内生真菌 次级代谢产物 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]