荧光金纳米簇的制备及对铜离子的检测应用  

Rapid and Highly Selective Detection of Copper Ion With Fluorescent Gold Nanoclusters

作　　者：胡娅琪 马瑞龙 陈庆庆 张博[1] 姚琳[1] 秦蓓[1] HU Ya-qi;MA Rui-long;CHEN Qing-qing;ZHANG Bo;YAO Lin;QIN Bei(Institute of Drug Research,College of Pharmacy,Xi'an Medical College,Xi'an Shaanxi 7100211,China)

机构地区：[1]西安医学院药物研究所,西安医学院药学院,陕西西安710021

出　　处：《当代化工》2022年第5期1061-1065,共5页Contemporary Chemical Industry

基　　金：陕西省重点研发计划项目资助(2021ZDLSF03-05);陕西省教育厅项目(项目编号:20JK0892、21JK0894);西安医学院校级科技创新团队(项目编号:2021TD07)和西安医学院药学重点学科建设项目资助。

摘　　要：建立了一种基于牛血清蛋白(BSA)包裹的金纳米簇(BSA/AuNCs)高选择性检测水中Cu^(2+)的方法。探讨了BSA-Au NCs的合成条件,在p H=8、氯金酸浓度为10 mmol·L^(-1)、反应时间为24 h、反应温度为37℃的条件下,制备了发射红色荧光的BSA-Au NCs,避免了复杂生物基质背景荧光的干扰,可快速、灵敏、高选择性地检测Cu^(2+),荧光强度的改变值与Cu^(2+)浓度在20~550μmol·L^(-1)范围内具有良好的线性关系,检出限为5.6μmol·L^(-1)。测得水样中Cu^(2+)的加标回收率为96.3%~99.5%,有望应用于环境监测、临床诊断等方面。A highly selective method for the determination of Cu^(2+)based on Au NCs coated with bovine serum albumin(BSA) was developed. Under the optimal conditions(p H=8, C(HAu Cl4)=10 mmol·L^(-1), reaction time=24 h, and reaction temperature =37 ℃), BSA-Au NCs with emitting red light was prepared, which avoided the interference of background fluorescence of complex biomaterial. Cu^(2+)can be detected rapidly, sensitively and selectively, the linear rage of Cu2+ was from 20 to 550 μmol·L^(-1)with a detection limit of 5.6 μmol·L^(-1). In addition, the reliability of Au NCs was also verified by detection of Cu^(2+)in water samples with satisfactory recoveries(96.3%~99.5%). This study provides a simple, fast and reliable method to detect Cu^(2+). The method is expected to be extended to the environmental monitoring and clinical diagnosis.

关 键 词：金纳米簇 铜离子 荧光分析法 

分 类 号：O657.3[理学—分析化学]