空肠弯曲菌CRISPR/Cas系统的研究进展  

Research progress of CRISPR/Cas system in Campylobacter jejuni

作　　者：吕泓玥 臧筱琦 黄萍瑜 焦新安 黄金林[1,2] LV Hongyue;ZANG Xiaoqi;HUANG Pingyu;JIAO Xin’an;HUANG Jinlin(Jiangsu Key Laboratory of Zoonosis,Yangzhou University,Yangzhou 225009,Jiangsu,China;Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for Agrifood Safety and Quality,Ministry of Agriculture and Rural Affairs of China,Yangzhou 225009,Jiangsu,China)

机构地区：[1]扬州大学江苏省人兽共患病学重点实验室,江苏扬州225009 [2]农业农村部农产品质量安全生物性危害因子(动物源)控制重点实验室,江苏扬州225009

出　　处：《微生物学报》2022年第7期2455-2465,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(32172939,31872493);山东省泰山产业领军人才项目(tscy20190113)。

摘　　要：多数细菌都存在发挥免疫防御机制作用的CRISPR/Cas系统,在不同种属间呈现多态性。空肠弯曲菌是全球范围内重要的食源性病原菌,所致疾病也是典型的自限性疾病,其复杂的致病机制并未得到明确的解析,而空肠弯曲菌CRISPR/Cas系统的结构呈现多态性,研究两者关系仍存在诸多限制。本文从CRISPR/Cas系统在空肠弯曲菌中的结构、机制及技术应用等方面的研究进展进行综述,为探索空肠弯曲菌致病机制提供新思路。CRISPR/Cas system is distributed in most bacteria,with immune defense mechanisms and showing polymorphisms among different species.Campylobacter jejuni is an important foodborne pathogen worldwide,and the disease caused by infection with it is also a typical self-limiting disease.Its pathogenic mechanism is complex,which has not been clearly clarified,and together with the polymorphisms of CRISPR/Cas system in C.jejuni,there exist many limitations in analyzing the relationship between CRISPR/Cas system and C.jejuni.In this paper,the structure,mechanism and technical application of CRISPR/Cas system in C.jejuni were reviewed,which provided new ideas for exploring the pathogenic mechanism of C.jejuni.

关 键 词：空肠弯曲菌 CRISPR/Cas系统 结构 免疫防御 

分 类 号：Q78[生物学—分子生物学] R378[医药卫生—病原生物学]