长非编码RNA linc00641通过调节miR-362-5p/NDRG1逆转前列腺癌细胞多西他赛耐药的研究  

Study on reversal of docetaxel resistance in prostate cancer cells through regulation of miR-362-5p/NDRG1 by long noncoding RNA linc00641

作　　者：郭晓波[1] 李岗[1] 赵宇峰[1] 赵波 GUO Xiaobo;LI Gang;ZHAO Yufeng;ZHAO Bo(Department of Urology,Heji Hospital Affiliated to Chcmgzhi Medical College,Changzhi 046011,China)

机构地区：[1]长治医学院附属和济医院泌尿外科,山西长治046011

出　　处：《沈阳药科大学学报》2022年第6期703-708,共6页Journal of Shenyang Pharmaceutical University

基　　金：山西省教育厅课题(2019L0697)。

摘　　要：目的研究长非编码RNA linc00641对前列腺癌细胞多西他赛(docetaxel,DTX)耐药的影响,并探究可能机制。方法取对数期PC-3细胞,分别转染pc-DNA与pc-DNA-linc00641,设为对照组、实验组。取未处理细胞设为空白组。转染48 h后采用qRT-PCR法检测细胞linc00641表达量;MTT法检测DTX对细胞的半数抑制浓度(IC_(50));流式细胞术检测细胞凋亡率;qRT-PCR检测细胞miR-362-5p表达量;免疫印迹法检测细胞N-myc下游调节基因1(NDRG1)蛋白表达量。结果与空白组、对照组比较,实验组linc00641表达量、不同浓度DTX对细胞的增殖抑制率、细胞凋亡率、NDRG1蛋白表达量升高(P<0.05),DTX对细胞的IC_(50)、miR-362-5p表达量降低(P<0.05)。结论过表达linc00641可逆转前列腺癌细胞DTX耐药,可能与其靶向下调miR-362-5p进而上调NDRG1表达有关。Objective To study the effect of long non-coding RNA linc00641 on the resistance of prostate cancer cells to docetaxel(DTX)and explore the possible mechanism.Methods PC-3 cells in logarithmic phase were transfected with pc-DNA and pc-DNA-linc00641 respectively,and set as control group and experimental group,and untreated cells was used as the blank group.The expression of linc00641 were detected by qRT-PCR method after transfection for 48 h.MTT method was used to detect the half inhibitory concentration(IC_(50))of DTX on cells.The apoptosis rate were detected by flow cytometry.The expression of miR-362-5 p was detected by qRT-PCR method.The expression of N-myc downstream regulatory gene 1(NDRG1)protein was detected by Western blotting.Results Compared with the blank group and the control group,the expression of linc00641,the proliferation inhibition rate,apoptosis rate and NDRG1 protein expression of different concentrations of DTX in the experimental group were increased(P<0.05),the IC_(50)of DTX on cells and the miR-The expression of miR-362-5 p were decreased(P<0.05).Conclusion Overexpression of linc00641 can reverse the resistance of prostate cancer cells to DTX,which may be related to the down-regulation of miR-362-5 p and the up-regulation of NDRG1 expression.

关 键 词：前列腺癌 多西他赛 耐药 增殖 凋亡 

分 类 号：R697[医药卫生—泌尿科学]