基于分子生物学技术对四川省石渠县野外犬科动物蠕虫感染情况调查  

Investigation of helminth infection in wild canines in Shiqu County,Sichuan Province based on molecular biology technology

作　　者：李春阳 王旭 陈齐鲁 付梅花 刘白雪 伍卫平 官亚宜 LI Chun-yang;WANG Xu;CHEN Qi-lu;FU Mei-hua;LIU Bai-xue;WU Wei-ping;GUAN Ya-yi(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),NHC Key Laboratory of Parasite and Vector Biology,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Shanghai 200025,China;Shanghai Municipal Center for Disease Control and Prevention)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)国家卫生健康委员会寄生虫病原与媒介生物学重点实验室世界卫生组织热带病合作中心国家级热带病国际联合研究中心,上海200025 [2]上海市疾病预防控制中心

出　　处：《中国病原生物学杂志》2022年第6期675-681,共7页Journal of Pathogen Biology

基　　金：上海市卫生健康委员会科研项目(No.20214Y0213)。

摘　　要：目的采用分子生物学技术检测四川省石渠县野外犬科动物蠕虫感染情况,为当地人畜共患寄生虫的防控提供参考。方法于2021年5月在四川省石渠县色须镇野外环境中收集新鲜犬科动物粪便,提取DNA,通过PCR扩增核糖体的18srna基因、线粒体的cox1基因和nad1基因进行虫种鉴定,并计算野外犬科动物蠕虫检出率。结果石渠县野外犬科动物粪便中蠕虫检出率为36.43%(47/129)。虫种检出率分别为:狭首钩刺线虫(Uncinaria stenocephala)27.13%(35/129),犬弓首蛔虫(Toxocara canis)6.98%(9/129)、猪蛔虫(Ascaris suum)3.10%(4/129),狐环体线虫(Crenosoma vulpis)1.55%(2/129),有齿冠尾线虫(Stephanurus dentatus)0.78%(1/129),石渠棘球绦虫(Echinococcus shiquicus)4.65%(6/129),田氏带绦虫(Taenia tianguangfui)和线形中殖孔绦虫(Mesocestoides lineatus)0.78%(1/129)。虫种检出率差异具有统计学意义(χ^(2)=133.730,P<0.05)。129份犬粪样品中有12份检出两种以上蠕虫。3种通用引物对线虫的检出率依次为8.53%(11/129),28.68%(37/129)和13.18%(17/129),差异具有统计学意义(χ^(2)=20.561,P<0.05);通用引物和特异性引物对石渠棘球绦虫的检出率分别为0和4.65%,差异具有统计学意义(χ^(2)=4.266,P<0.05)。结论四川省石渠县野外犬科动物蠕虫感染普遍,检出率较高,需加强对当地狭首钩刺线虫、犬弓首蛔虫、石渠棘球绦虫等人畜共患寄生虫的防控。扁形动物门通用引物在检测带绦虫感染时的检出效率较低,需要研发更为高效的带绦虫检测通用引物。Objective The worm infection of wild canines in Shiqu county,Sichuan Province was detected by molecular biology technique,which provided reference for the prevention and control of zoonotic parasites in the local area.Methods In May,2021,fresh canine feces samples were collected in the wild environment of Sexu Town,Shiqu County,Sichuan.DNA was extracted from stool samples,and the 18srna gene of ribosome,cox1 gene and nad1 gene of mitochondria were amplified by PCR,then the insect species were identified,and the positive rate of the worm infection of canine in the field was calculated.Results The positive rate of the worm detected in the feces of wild canines in Shiqu County was 36.43%(47/129).The worm detection rates were 27.13%(35/129)for Uncinaria stenocephala,6.98%(9/129)for Toxocara canis,3.10%(4/129)for Ascaris suum,1.55%(2/129)for Crenosoma vulpis,and 0.78%(1/129)for Stephanurus dentatus,4.65%(6/129)for Echinococcus shiquicus,0.78%(1/129)for Taenia tianguangfui and 0.78%(1/129)for Mesocestoides lineatus,respectively.The difference in detection rate among worm species was statistically significant(χ~2=133.730,P<0.05).Among the 129 samples,12 samples were mixed with two or more worms.The detection rates of the three universal primer pairs in Caenorhabditis elegans were 8.53%(11/129),28.68%(37/129)and 13.18%(17/129),and the difference between them was statistically significant(χ~2=20.561,P<0.05).The detection rates of Echinococcus shiquicus by universal primer and specific primer were 0%and 4.65%respectively,and the difference was statistically significant(χ~2=4.266,P<0.05).Conclusion In Shiqu County,Sichuan province,wild canine helminth infection is common and the positive rate of the detection rate is high.It is necessary to strengthen the prevention and control of local zoonotic parasites such as U.stenocephala,T.canis,and E.shiquicus.The detection efficiency of the universal primers for the Platyhelminthes in the detection of Taeniata species infection is poor,and it is necessary to continue to explore

关 键 词：蠕虫 狭首钩刺线虫 犬弓首蛔虫 石渠棘球绦虫 野外犬科动物 

分 类 号：R383[医药卫生—医学寄生虫学]