慢阻肺患者咽部菌群失衡与白细胞介素簇及CD4^(+)/CD8^(+)水平的相关性  

Correlation of pharyngeal flora imbalance with interleukin cluster and CD4^(+)/CD8^(+) values in patients with chronic obstructive pulmonary disease

作　　者：赵慧[1] 武宏[2] 赵丽洁[1] 李文娟 ZHAO Hui;WU Hong;ZHAO Li-jie;LI Wen-juan(School of Medicine and Hygiene,Anyang Vocational and Technical College,Anyang,Henan 455000,China;Department of Brain Disease,Anyang Traditional Chinese Medicine Hospital;Department of Intensive Care Unit,Anyang People’s Hospital)

机构地区：[1]安阳职业技术学院医药卫生学院,河南安阳455000 [2]安阳市中医院脑病科 [3]安阳市人民医院重症监护室

出　　处：《中国病原生物学杂志》2022年第6期714-717,共4页Journal of Pathogen Biology

摘　　要：目的 分析慢阻肺患者咽部菌群失衡与白细胞介素簇及CD4^(+)/CD8^(+)水平的相关性。方法 选取2019年1月-2021年3月安阳市人民医院收治的慢阻肺患者125例为慢阻肺组,另选取同期进行体检的健康者80例,为健康组。采集两组受试者的咽拭子标本进行细菌培养,观察咽部菌群分布。采用酶联免疫吸附法检测受试者血清白介素IL-4、IL-8、IL-10水平,流式细胞仪检测CD4^(+)、CD8^(+),并计算CD4^(+)/CD8^(+)值。采用Pearson相关分析法分析咽部菌群数量与白细胞介素及CD4^(+)/CD8^(+)值的关系。结果 慢阻肺组患者咽部检出菌群株数及主要致病菌所占比例显著高于健康组(均P<0.05),检出的主要致病菌数量居前3位的是凝固酶阴性葡萄球菌、大肠埃希菌和金黄色葡萄球菌,分别占28.00%、27.20%和22.40%。慢阻肺组患者血清IL-4、IL-8水平显著高于健康组(P<0.05),IL-10水平显著低于健康组(P<0.05),CD4^(+)百分率及CD4^(+)/CD8^(+)值显著低于健康组(P<0.05),CD8^(+)水平与健康组差异无统计学意义(P>0.05)。经Pearson相关性分析,慢阻肺患者咽部菌群数量与血清IL-4、IL-8水平呈正比(P<0.05),与血清IL-10水平及CD4^(+)/CD8^(+)值呈反比(P<0.05)。结论 慢阻肺患者咽部菌群失衡与体内白细胞介素簇、CD4^(+)/CD8^(+)比值密切相关,临床上应及时监测患者血清上述指标评估病情,采取相应的治疗措施。Objective To investigate the correlation of pharyngeal flora imbalance with interleukin cluster and CD4^(+)/CD8^(+) values in patients with chronic obstructive pulmonary disease(COPD).Methods One hundred and twenty-five patients with COPD in Anyang People’s Hospital from January 2019 to March 2021 were enrolled,and another 80 healthy individuals underwent physical examination in our hospital were set as healthy group.Bacterial cultures of throat swabs were performed in both groups to detect the distribution of pharyngeal flora,and enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of interleukins(IL-4,IL-8,and IL-10) in venous blood.CD4^(+) and CD8^(+) contents were determined by flow cytometry,and CD4^(+)/CD8^(+) ratio was calculated.Then the correlation of pharyngeal flora with interleukin cluster and CD4^(+)/CD8^(+)was discussed using Pearson correlation analysis.Results The proportion of main pathogenic bacteria in pharynx of COPD group was significantly higher than that of healthy group(P0.05).Pearson correlation analysis showed that the number of pharyngeal bacteria was positively correlated with serum IL-4 and IL-8 levels(P<0.05),but negatively correlated with serum IL-10 levels and CD4^(+)/CD8^(+) ratio(P<0.05).Conclusion The imbalance of pharyngeal flora in patients with COPD is closely related to the interleukin cluster and CD4^(+)/CD8^(+) ratio,so the monitoring of the above indexes is of great significance in the clinical diagnosis and the establishment of appropriate treatment measures.

关 键 词：慢阻肺 咽部菌群 白细胞介素簇 T淋巴细胞亚群 相关性 

分 类 号：R378[医药卫生—病原生物学]