白头翁皂苷B_(4)栓剂治疗大鼠痔疮的药效学研究  

Study on pharmacodynamics of anemoside B_(4) suppository in treatment of rats with hemorrhoid

作　　者：张琛伟 许琼明[2,3] 高红伟 苑仁祎坤 杨世林 ZHANG Chenwei;XU Qiongming;GAO Hongwei;YUAN Renyikun;YANG Shilin(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530020,China;College of Pharmaceutical Science,Soochow University,Suzhou 215123,China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)

机构地区：[1]广西中医药大学药学院,广西南宁530020 [2]苏州大学药学院,江苏苏州215123 [3]天然药物活性物质与功能国家重点实验室,中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《药物评价研究》2022年第5期853-863,共11页Drug Evaluation Research

基　　金：2020年度天然药物活性与功能国家重点实验室研究课题(GTZK202007)。

摘　　要：目的 探究白头翁皂苷B栓剂对冰醋酸、巴豆油及细菌所致痔疮模型大鼠的治疗作用。方法 应用混合脂肪酸甘油酯36型号和38型号制备含白头翁皂苷B5、10、20 mg·粒^(-1)的栓剂。分别用冰醋酸、巴豆油、细菌建立急性痔疮大鼠模型,每种模型制备完成后大鼠均随机分为模型组、空白栓剂组、马应龙麝香痔疮膏(阳性药,1 g·kg^(-1))组和白头翁皂苷B栓剂(5、10、20 mg·粒^(-1))组,每组10只,对照组未经任何处理。白头翁皂苷B栓剂各剂量组每天给药2次,给药3 d,共6次;马应龙麝香痔疮膏每天1次,给药3 d,共3次。3 d后取大鼠肛门组织,HE染色后比较各组大鼠肛周组织病理形态学变化;血常规检测仪检测血液中白细胞(WBC)、中性粒细胞(NEUT)、淋巴细胞(LYMPH)水平;ELISA法检测大鼠血清和肛门组织中白细胞介素-6(IL-6)、IL-1β、肿瘤坏死因子-α(TNF-α)水平。结果 与模型组比较,白头翁皂苷B栓剂明显改善大鼠肛周组织充血肿胀及肛周组织病变,显著降低血液中WBC、LYMPH和NEUT水平,显著抑制血清和组织中IL-6、IL-1β和TNF-α水平(P<0.05、0.01、0.001)。结论 白头翁皂苷B栓剂能有效改善急性痔疮模型大鼠的组织病变,调节免疫细胞,抑制炎症因子反应,对急性痔疮具有治疗作用。Objective To explore the therapeutic effect of anemoside Bsuppository on hemorrhoid model rats caused by acetic acid,croton oil and bacteria. Methods Anemoside Bsuppository of 5, 10 and 20 mg·granule^(-1) was prepared by mixed fatty acid glyceride36 and 38 models. Three models of hemorrhoids were prepared with acetic acid, croton oil, and bacteria. After successful modeling,rats were randomly divided into model group, blank suppository group, Mayinglong Musk hemorrhoid ointment(positive drug, 1 g·kg^(-1))group and anemoside Bsuppository 5, 10, 20 mg·granule^(-1) groups, ten in each group and rats control group without any treatment.Anemoside Bsuppository was administered twice a day for 3 d, 6 times in total. Mayinglong musk Hemorrhoid ointment was given once a day for 3 d, 3 times in total. Anal tissues of rats were collected 3 d later, and the pathological changes of perianal tissues were compared after HE staining. Blood routine examinations were performed to detect the levels of white blood cells(WBC), NEUT and LYMPH. The levels of interleukin-6(IL-6), IL-1β and tumor necrosis factor-α(TNF-α) in serum and anal tissue of rats were determined by ELISA. Results Compared with model group, anemoside Bsignificantly improved rat perianal tissue congestion and swelling and perianal tissue lesions, reduced the levels of WBC, NEUT, and LYMPH in the blood, and inhibited the inflammatory cytokines IL-6, IL-1β and TNF-α in serum and tissues(P < 0.05、0.01、0.001). Conclusion Anemoside Bcan effectively treat anal tissue lesions in rats with acute hemorrhoids, and its mechanism of action may be related to reducing the levels of WBC, NEUT, and LYMPH and inhibiting the release of inflammatory cytokines IL-6, IL-1β and TNF-α.

关 键 词：痔疮 白头翁皂苷B_(4) 栓剂 病理组织形态 免疫细胞 炎症因子 

分 类 号：R285.5[医药卫生—中药学]