基于路径分析数学模型探讨逍遥散功效药队抗抑郁配伍关系  

Study on compatibility of anti-depression effects of Xiaoyaosan’s efficacy groups based on path analysis mathematical model

作　　者：渐衬 李孟宇 王雅泽 吕梦 李顺勇[4] 陈士剑 秦雪梅[1,2,3] 刘晓节 JIAN Chen;LI Mengyu;WANG Yaze;Lü Meng;LI Shunyong;CHEN Shijian;QIN Xuemei;LIU Xiaojie(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;Institute of Biomedicine and Health,Shanxi University,Taiyuan 030006,China;Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Taiyuan 030006,China;School of Mathematical Sciences,Shanxi University,Taiyuan 030006,China;School of Computer and Information Technology,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学生物医药与大健康研究院,山西太原030006 [3]化学生物学与分子工程教育部重点实验室,山西太原030006 [4]山西大学数学科学学院,山西太原030006 [5]山西大学计算机与信息技术学院,山西太原030006

出　　处：《药物评价研究》2022年第5期871-877,共7页Drug Evaluation Research

基　　金：国家自然科学基金青年项目(81803962);山西省留学回国人员科技活动择优资助项目(20200013)。

摘　　要：目的 采用路径分析数学模型定量表征逍遥散功效药队(健脾药队、疏肝药队)对整方抗抑郁药效的贡献。方法 大鼠适应性饲养1周后,基于行为学数据以及体质量,随机分为对照组、模型组、疏肝(25 g·kg^(-1),柴胡、薄荷、白芍、当归)组、健脾(21.3 g·kg^(-1),白术、茯苓、生姜、甘草)组、逍遥散(46.3 g·kg^(-1),柴胡、当归、白芍、白术、茯苓、甘草、薄荷、生姜质量比为6∶6∶6∶6∶6∶3∶2∶2)组,制备慢性温和不可预知应激(CUMS)抑郁大鼠模型同时给药。对照组和模型组给予等量生理盐水,在给药1 h后开始造模,每天给药1次,持续28 d。在第0、1、2、3、4周分别对所有动物进行称体质量、糖水偏爱实验;在造模结束后对大鼠进行强迫游泳和水迷宫行为学测试。应用IBM SPSS Statistics 26软件,处理、分析行为学数据,并绘制路径分析效果图。结果 造模第4周,模型组大鼠体质量、糖水偏爱率、水迷宫穿越平台次数显著低于对照组(P<0.05),强迫游泳不动时间显著高于对照组(P<0.05)。与模型组比较,各给药组体质量、穿越平台次数显著增加(P<0.05、0.01),其中逍遥散组效果最优,健脾组效果次之,最后为疏肝组;各给药组糖水偏爱率显著增加(P<0.01),逍遥散的作用优于健脾组和疏肝组,与对照组最为接近;各给药组不动时间显著减少(P<0.05、0.01),健脾的效果优于逍遥散和疏肝组。路径分析结果 显示,体质量:疏肝药队对整方的作用是促进作用,但健脾药队具有抑制作用,疏肝药队的正向作用强于健脾药队的抑制作用;水迷宫实验:疏肝药队和健脾药队均发挥正向促进作用;糖水偏爱率:健脾药队对整方的贡献是正向促进作用,但疏肝药队是抑制作用,健脾药队的正向促进作用强于疏肝药队的抑制作用;强迫游泳:健脾药队的作用是正向促进的,但疏肝药队是抑制作用,疏肝的抑制作用强于健脾的正向作Objectives To explore the application of path analysis in the study of the compatibility of traditional Chinese medicine(TCM) prescriptions, we used the path analysis mathematical model to quantitatively characterize the contributions of Xiaoyaosan’s efficacy groups, i.e. the antidepressant efficacy of the Shugan group and the Jianpi group in the whole prescription. Methods After one week of adaptive feeding, the rats were randomly divided into control group, model group, Shugan group(25 g·kg^(-1)), Jianpi group(21.3 g·kg^(-1)) group, and Xiaoyaosan(46.3 g·kg^(-1)) group based on behavioral data and body weight. Rats in control group and the model group were given the same amount of normal saline. The model was established 1 h later. The model was administered once a day for 28 d. At the 0, 1, 2, 3 and 4 weeks, all animals were tested for weight and sugar preference. Forced swimming and water maze behavior tests were performed on the rats after modeling. Finally, the IBM SPSS Statistics 26 software was used to proceed and analyze the behavioral data. Besides, a path analysis effect diagram was drawn. Results At the 4th week of modeling,the body weight, sugar preference rate and times of water maze crossing platform in model group were significantly lower than those in control group(P < 0.05), while the immobility time of forced swimming was significantly higher than that in control group(P <0.05). Compared with model group, body weight and times of crossing platform in each administration group were significantly increased(P < 0.05, 0.01). Xiaoyaosan group exhibited the best effect, followed by Jianpi group, and finally was Shugan group. The sugar preference rate of each administration group increased significantly(P < 0.01), and the effect of Xiaoyaosan was stronger than that of Jianpi group and Shugan group, which was closest to the control group. The immobile time of each administration group was significantly decreased(P < 0.05, 0.01). The results of path analysis showed that body weight: the effect of S

关 键 词：路径分析 逍遥散 功效药队 健脾药队 疏肝药队 配伍 抑郁症 

分 类 号：R285.5[医药卫生—中药学]