鸽新城疫病毒Ⅵ-HZ株的分离鉴定及其遗传进化分析  

Isolation,identification and genetic evolution analysis of pigeon Newcastle disease virusⅥ-HZ strain

作　　者：钱晶[1] 刘雪 王玮 周红波[3] 王永山[1] QIAN Jing;LIU Xue;WANG Wei;ZHOU Hongbo;WANG Yongshan(Institute of Veterinary Medicine,Key Laboratory of Veterinary Biological Engineering and Technology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Animal Science and Technology,Guangdong Laboratory for Lingnan Modern Agriculture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;College of Veterinary Medicine,State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室,江苏南京210014 [2]仲恺农业工程学院动物科技学院岭南现代农业科学与技术广东省实验室,广东广州510225 [3]华中农业大学动物医学院农业微生物学国家重点实验室,湖北武汉430070

出　　处：《中国兽医学报》2022年第4期651-656,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32002274);广东省重点领域研发计划资助项目(2020B0202080002);江苏省自然科学基金资助项目(BK20180299);江苏省农业科技自主创新资金资助项目(CX(19)3019)。

摘　　要：对疑似鸽新城疫(Newcastle disease,ND)的病料进行病毒鉴定和分离培养,获得1株鸽新城疫病毒(Newcastle disease virus,NDV)Ⅵ-HZ株,为进一步了解病毒生物学特性及其分子特征,对其进行致病性和遗传进化分析。结果表明,该毒株MDT为80 h,ICPI为1.2,EID_(50)为10^(7.0)/0.1 mL,HA效价为8 log_(2),属于中等毒力型NDV;人工感染鸽第7天出现明显腹泻和神经症状,死亡率为60%,病死鸽剖检可见脑膜充血、水肿及肠道出血;病理切片结果显示病死鸽脑组织水肿、小肠绒毛上皮细胞脱落坏死、黏膜固有层有炎症。全基因组测序显示Ⅵ-HZ株全长为15192 nt,基因排列方式为3'-NP-P/V/W-M-F-HN-L-5',其中F蛋白裂解位点氨基酸序列为^(112)KRQKRF^(117),具有NDV强毒的分子特征;系统发育进化分析表明该Ⅵ-HZ株为ClassⅡ类基因Ⅵ型毒株,与Ⅵ.2.2.2毒株亲缘关系最近。本研究为近期鸽NDV的分子流行病学调查提供了科学依据。A pigeon Newcastle disease virus(NDV)Ⅵ-HZ strain was isolated and identified from the sample of suspected Newcastle disease(ND).To further understand the biological characteristics and molecular characteristics ofⅥ-HZ strain,virus pathogenicity and genetic evolution were analyzed.The results showed that the values of MDT,ICPI,EID_(50) and Hemagglutinin(HA)are 80 h,1.2,10^(7.0)/0.1 mL and 8 log_(2),belonging to mesogenic NDV.The artificially infected pigeons showed obvious diarrhea and neurological symptoms at 7 days post-infection(dpi),and the mortality rate was 60%.The experimental pigeon autopsy showed meningeal congestion,edema and intestinal bleeding.The histology results showed brain edema,necrosis of the villous epithelial cell loss and inflammation of lamina propria.The complete genome of Ⅵ-HZ strain is 15192 nucleotides(nts)with the gene order of 3'-NP-P/V/W-M-F-HN-L-5'.The Ⅵ-HZ strain contains the ^(112)KRQKRF^(117) cleavage site in F protein,a feature generally associated with virulent NDV strains.The phylogenetic tree of full F gene sequence showed that Ⅵ-HZ strain was closely related to Class Ⅱ genotype Ⅵ NDV,and closest relationship with genotype Ⅵ.2.2.2strain.This study provides a basis for the recent molecular epidemiological investigation of pigeon NDV.

关 键 词：鸽新城疫病毒 致病性 遗传进化 F基因 基因Ⅵ型 

分 类 号：S852.659.2[农业科学—基础兽医学]