In vivo and in vitro development of Tibetan antelope(Pantholops hodgsonii)interspecific cloned embryos  

作　　者：Guanghua SU Lei CHENG Yu GAO Kun LIU Zhuying WEI Chunling BAI Fengxia YIN Li GAO Guangpeng LI Shorgan BOU 

机构地区：[1]The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology,Key Laboratory of Herbivore Reproductive Biotechnology and Breeding Ministry of Agriculture,Inner Mongolia University,Hohhot 010021,China

出　　处：《Frontiers of Agricultural Science and Engineering》2014年第1期21-29,共9页农业科学与工程前沿（英文版）

基　　金：supported by the National Basic Research Program of China(2012CB22306);the National Natural Science Foundation of China(Grant No.31372289).

摘　　要：The Tibetan antelope is endemic to the Tibetan Plateau,China,and is now considered an endangered species.As a possible rescue strategy,the development of embryos constructed by interspecies somatic cell nuclear transfer(iSCNT)was examined.Tibetan antelope fibroblast cells were transferred into enucleated bovine,ovine and caprine oocytes.These cloned embryos were then cultured in vitro or in the oviducts of intermediate animals.Less than 0.5%of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst stage.However,when the cloned antelope-bovine embryos were transferred to caprine oviducts,about 1.6%of the embryos developed to the blastocyst stage.In contrast,only 0.7%of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine oviducts.The treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and caprine.When the antelope-bovine embryos,constructed from oocytes treated with roscovitine or trichostatin A,were cultured in rabbit oviducts 2.3%and 14.3%developed to blastocysts,respectively.It is concluded that although some success was achieved with the protocols used,interspecies cloning of Tibetan antelope presents difficulties still to be overcome.The mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.

关 键 词：interspecific nuclear transfer BOVINE OVINE CAPRINE OVIDUCT apoptosis 

分 类 号：Q95[生物学—动物学]