Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost  

作　　者：Shujuan ZHANG Guoqi SONG Yulian LI Jie GAO Jiao WANG Guiju CHEN Haosheng LI Genying LI Zhendong ZHAO 

机构地区：[1]Crop Research Institute,Shandong Academy of Agricultural Sciences,Key Laboratory of Wheat Biology&Genetic Improvement on North Yellow&Huai River Valley,Ministry of Agriculture of the People Republic of China,National Engineering Laboratory for Wheat&Maize,Jinan 250100,China

出　　处：《Frontiers of Agricultural Science and Engineering》2014年第1期30-38,共9页农业科学与工程前沿（英文版）

基　　金：supported by Science&Technology Development Plan of Shandong Province(2013GNC11025);Shandong Agriculture and Seed Industry(2012);Funding for the Post-doctoral Innovative Projects of Shandong Province(201203024);the National Transgenic Major Project(2013ZX08002-004);China Agriculture Research System(CARS-03-1-08);Shandong Agriculture Research System,the national key technology R&D program of China(2011BAD35B03).

摘　　要：Spring radiation frost(SRF)is a severe environmental stress which impairs wheat yield and productivity worldwide.To better understand the mechanism of wheat(Triticum aestivum)responding to SRF,a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively.A total of 43 differentially expressed proteins(DEPs)which mainly involved in carbohydrate metabolism,amino acid metabolism,resistance proteins and antioxidant enzymes,photosynthesis and cellular respiration proteins,cell-wall related proteins,protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS.The results revealed that of the 21 DEPs in Jimai22 responding to the SRF,13 DEPs were upregulated and 8 DEPs were downregulated,and that of the 22 DEPs in Luyuan301,9 DEPs were upregulated and 13 DEPs were downregulated.These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301.The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.

关 键 词：common wheat spring radiation frost proteomic analysis 2-DE MALDI-TOF-TOF MS 

分 类 号：S51[农业科学—作物学]