基于中药Q-marker理论的经典名方枳实薤白桂枝汤关键成分发现与量质传递研究  

Study on the Discovery of the Index Components and Quantitative-qualitative Transfer of Classical Prescription Zhishi Xiebai Guizhi Decoction Based on Q-marker

作　　者：李明潺[1] 杨红素 徐家山 曲珍妮 段宏泉[3] 董林毅[3] 孙露 LI Ming-chan;YANG Hong-su;XU Jia-shan;QU Zhen-ni;DUAN Hon-quan;DONG Lin-yi;SUN Lu(General Hospital,Tianjin Medical University,Tianjin 300052,China;Tonghua Yu Sheng Pharmaceutical Co.,Ltd.,Tonghua 134000,China;College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;NHC Key Laboratory of Hormones and Development/Tianjin Key Laboratory of Metabolic Diseases,Chu Hsien-I Memorial Hospital/Tianjin Institute of Endocrinology,Tianjin Medical University,Tianjin 300134,China)

机构地区：[1]天津医科大学总医院,天津300052 [2]通化玉圣药业有限公司,吉林通化134000 [3]天津医科大学药学院,天津300070 [4]天津医科大学朱宪彝纪念医院天津市内分泌研究所国家卫健委激素与发育重点实验室天津市代谢性疾病重点实验室,天津300134

出　　处：《化学试剂》2022年第8期1097-1102,共6页Chemical Reagents

基　　金：中药经典名方物质基准及颗粒剂开发校企合作项目(KY2020055)。

摘　　要：枳实薤白桂枝汤收录于国家经典名方目录,中药质量标志物(Q-marker)理论有助于方剂中关键成分的发现与量质传递研究。采用液质联用技术指认枳实薤白桂枝汤中的Q-marker,进行指纹图谱方法学考察和相似度分析,计算方剂出膏率和关键成分的含量转移率。最终指认大麦芽碱、麦角甾苷、新北美圣草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、异米拉素、苜蓿素、厚朴酚、和厚朴酚为枳实薤白桂枝汤Q-marker,18批样品指纹图谱相似度分析结果均大于0.92,平均出膏率为17.06%,关键成分的转移率以腺苷、辛弗林、桂皮醛、厚朴酚、和厚朴酚计算分别为87.49%、79.95%、25.04%、6.18%、8.80%。实验确定了枳实薤白桂枝汤质量标志物,结合指纹图谱及关键成分的量质传递研究,为枳实薤白桂枝汤质量评价提供了依据。Zhishi Xiebai Guizhi Decoction was included in the catalog of national classic prescriptions.Q-marker theory was helpful in the discovery of the index components and quantitative-qualitative transfer study in Chinese herbal prescriptions.Q-marker in Zhishi Xiebai Guizhi Decoction was identified by LC-MS and the fingerprint methodology was investigated,and the similarity analysis was evaluated.The paste-forming rate and the transfer rate of index components were also calculated.Q-marker were identified as follows:Hordenine,Verbascoside,Neoeriocitrin,Narirutin,Naringin,Hesperidin,Neohesperidin,Isoramil,Tricin,Honokiol,Magnolol.The similarity of 18 batches of Zhishi Xiebai Guizhi Decoction samples was more than 0.92.The average paste-forming rate was 17.06%.The transfer rates of adenosine,synephrine,cinnamaldehyde,honokiol,and honokiol were 87.49%,79.95%,25.04%,6.18%,and 8.80%,respectively.The Q-markers of Zhishi Xiebai Guizhi Decoction were determined.Combined with the fingerprint and the quantitative-qualitative transfer of index components,it provided a basis for the quality evaluation of classical prescription Zhishi Xiebai Guizhi Decoction.

关 键 词：枳实薤白桂枝汤 液质联用 指纹图谱 中药质量标志物 经典名方 

分 类 号：R286.02[医药卫生—中药学]