小叶黄杨的化学成分及心肌细胞保护作用研究  

Chemical Constituents and Cardioprotective Effects of Buxus Sinica var.parvifolia M.Cheng

作　　者：王瑞 张岩 MUHAMMAD Ishaq 田艳 付登林 郁华军 肖雪 严诗楷[1,2,3] 金慧子 WANG Rui;ZHANG Yan;MUHAMMAD Ishaq;TIAN Yan;FU Deng-lin;YU Hua-jun;XIAO Xue;YAN Shi-kai;JIN Hui-zi(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China;Guizhou Jingcheng Pharmaceutical Co.,Ltd.,Guiyang 550200,China;Institute of Chinese Medicinal Sciences,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]上海交通大学药学院,上海200240 [2]贵州景诚制药有限公司,贵州贵阳550200 [3]广东药科大学中医药研究院,广东广州510006

出　　处：《化学试剂》2022年第8期1111-1117,共7页Chemical Reagents

基　　金：国家自然科学基金项目(81973191);贵州省科技计划项目(【2018】2831)。

摘　　要：研究小叶黄杨(Buxus sinica var.parvifolia M.Cheng)叶子的化学成分及心肌细胞保护作用。综合运用硅胶、Sephadex LH-20、MCI、半制备液相等多种色谱分离方法,对小叶黄杨中等极性部位进行分离纯化,采用波谱学技术对分离的单体化合物进行结构鉴定。另外,对提取各部位和分离得到的14个化合物进行体外心肌细胞保护作用测试。从小叶黄杨干燥叶中共分离得到14个化合物,分别为香草酸乙酯(1)、香草醛(2)、花椒毒素(3)、反式对羟基肉桂酸乙酯(4)、3,4,5-trimethoxycinnamyl alcohol(5)、丁香酸乙酯(6)、(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5 H)-one(7)、桦木酸(8)、4-羟基-3,5-二甲氧基苯甲醛(9)、7,3′,4′-三甲基槲皮素(10)、白桦脂醇(11)、penduletin(12)、23-O-(E)-p-coumaroyl-23-hydroxybetulin(13)、松脂素(14)。其中化合物2、5和6为首次从该植物中分离得到,体外实验表明EA部位和化合物14(松脂素)可显著改善H_(2)O_(2)诱导的心肌细胞损伤。The chemical constituents from the leaves of Buxus Sinica var.parvifolia M.Cheng and their cardioprotective bioactivities were studied.The chemical constituents from the ethyl acetate(EA)part of this plant were isolated and purified by column chromatography such as silica gel,Sephadex LH-20,MCI,and preparative HPLC.The structures of isolates were elucidated by spectroscopic analyses.A hydrogen peroxide(H_(2)O_(2))-induced cardiomyocyte line H9c2 was used as an in vitro model of damaged cardiomyocytes to evaluate the effects of the extracts and the isolates.Fourteen compounds were isolated and elucidated as ethyl 4-hydroxy-3-methoxybenzoate(1),vanillin(2),xanthotoxin(3),trans-p-hydroxycinnamic acid ethyl ester(4),3,4,5-trimethoxycinnamyl alcohol(5),syringic acid ethyl ester(6),(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5 H)-one(7),betulinic acid(8),4-hydroxy-3,5-dimethoxybenzaldehyde(9),7,3′,4′-trimethylquercetin(10),betulin(11),Pendleton(12),23-O-(E)-p-coumaroyl-23-hydroxybetulin(13)and pinoresinol(14).Compounds 2,5,and 6 were firstly isolated from this plant.The EA part and compound 14(pinoresinol)showed significant protective effects against H_(2)O_(2)-induced cardiomyocyte injury in H9c2 cells.

关 键 词：小叶黄杨 化学成分 心肌保护作用 H9C2细胞 

分 类 号：O629.9[理学—有机化学]