基于氧化石墨烯和三链DNA的三聚氰胺检测研究  

Study on Melamine Detection Based on Graphene Oxide and DNA Triplex Structure

作　　者：张丽媛 范淼 卢宏飞 张廉奉[1] 邢小静 ZHANG Li-yuan;FAN Miao;LU Hong-fei;ZHANG Lian-feng;XING Xiao-jing(College of Chemistry and Pharmaceutical Engineering,Nanyang Normal University,Nanyang 473061,China)

机构地区：[1]南阳师范学院化学与制药工程学院,河南南阳473061

出　　处：《化学试剂》2022年第8期1208-1212,共5页Chemical Reagents

基　　金：河南省科技攻关项目(202102110111,182102310646)。

摘　　要：发展成本低、准确度高、特异性好的三聚氰胺检测方法具有重要的研究意义。基于氧化石墨烯(GO)自身较好的荧光猝灭性能,结合含脱碱基位点的三链DNA和核酸外切酶Ⅲ的特殊性能,构建了一种高灵敏的特异性三聚氰胺检测新方法。首先制备了GO,并采用紫外-可见吸收光谱仪、傅里叶红外吸收光谱仪及透射电子显微镜对其进行了表征。在最佳的实验条件下,考察了设计方法对三聚氰胺的检测灵敏度和特异性。结果显示,体系荧光强度与三聚氰胺浓度呈现较好的线性关系,线性范围为0.5×10^(-9)~7.0×10^(-9) mol/L,检测限为0.32×10^(-9) mol/L。此外,本方法对奶粉样品中三聚氰胺检测的回收率为92.8%~106.1%,表现出很好的实际应用潜力。It is of great significance to develop a method for melamine detection with low-cost,high accuracy,and good specificity.Based on the fluorescence quenching property of graphene oxide(GO),combined with the special properties of DNA triplex structure with the abasic site and exonucleaseⅢ,a novel platform for melamine detection was developed.GO was first successfully prepared and characterized using UV-visible absorption spectroscopy,Fourier transforms infrared spectroscopy,and transmission electron microscopy.Under the optimum conditions,the detection sensitivity and specificity were investigated.The results showed that the fluorescence intensity displayed a good linear relationship with melamine concentration in the range from 0.5×10^(-9) to 7.0×10^(-9) mol/L,and the detection limit was 0.32×10^(-9) mol/L.Moreover,the recovery of melamine spiked in milk powder was from 92.8%to 106.1%,indicating the potential of the proposed method in practical application.

关 键 词：三聚氰胺 氧化石墨烯 三链DNA 脱碱基位点 核酸外切酶Ⅲ 

分 类 号：O657.3[理学—分析化学]