热碱法提取制革污泥蛋白质及表征  

Proteins extraction and characterization from tannery sludge by thermal-alkaline

作　　者：顾海洋 翟世民 王冬 张丽平 付少海[1,2,3] GU Hai-yang;ZHAI Shi-min;WANG Dong;ZHANG Li-ping;FU Shao-hai(Jiangsu Engineering Research Center for Digital Textile Inkjet Printing,Wuxi 214122,China;Key Laboratory of Eco-Textiles(Jiangnan University),Ministry of Education,Wuxi 214122,China;National Manufacturing Innovation Center of Advanced Dyeing and Finishing Technology,Taian 271001,China)

机构地区：[1]江苏省纺织品数字喷墨印花工程技术研究中心,江苏无锡214122 [2]生态纺织教育部重点实验室(江南大学),江苏无锡214122 [3]国家先进印染技术创新中心,山东泰安271001

出　　处：《应用化工》2022年第6期1576-1580,1585,共6页Applied Chemical Industry

基　　金：国家重点研发计划(2018YFC1902100);江苏省自然科学基金项目(BK20190613)。

摘　　要：采用热碱处理制革污泥制得蛋白质水解液,通过酸化提取制革污泥蛋白质。结果表明,随着NaOH用量、时间、温度的增加,制革污泥蛋白质的提取率呈先增长后下降的趋势;制革污泥蛋白质最佳提取工艺条件:温度150℃,时间60 min,NaOH用量为9 g/L,制革污泥蛋白提取率为71.2%;温度、温度和时间的交互是影响制革污泥蛋白质提取率的最主要因素。制革污泥蛋白质的纯度为83.01%,总铬含量为1.5 mg/kg,无机盐和灰分含量较低,常见氨基酸含量占比31.09%,蛋白质分子量分布在5~35 kDa之间。The protein hydrolysates were prepared by the thermal-alkaline treatment of tannery sludges,and then the proteins of tannery sludge were successfully extracted by the acidification method.The results showed that the extraction rate of sludge proteins increased and then decreased with the increase of NaOH dosage,time and temperature.The optimum temperature,time and NaOH dosage for extracting sludge proteins were 150℃,60 min and 9 g/L respectively,resulting in the highest extraction rate of 71.2%.The interaction of temperature,temperature and time is the most important factor to affect the extraction rate of sludge proteins.The purity,total chromium concentration,common amino acid content of extracted tannery sludge proteins is 83.01%,1.5 mg/kg and 31.09%,respectively.In addition,the sludge proteins have a low content of inorganic salts and ashes.The molecular weight of tannery sludge proteins ranges from 5 to 35 kDa.

关 键 词：制革污泥 蛋白质 热碱提取 提取率 资源化 

分 类 号：TQ949[化学工程] X794[环境科学与工程—环境工程]