鹅皮肤毛囊中WNT6基因mRNA的表达分析  

Expression of WNT6 Gene mRNA in Feather Follicles of Goose Skin

作　　者：刘静 孙越 周宇轩[1] 李晟毅 杨薇 隋玉健[1] 孙永峰[1] LIU Jing;SUN Yue;ZHOU Yuxuan;LI Shengyi;YANG Wei;SUI Yujian;SUN Yongfeng(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《吉林农业大学学报》2022年第2期210-214,共5页Journal of Jilin Agricultural University

基　　金：吉林省重点科技研发计划项目(20180201034NY);吉林省中青年科技创新领军人才及团队项目(2020-2022);吉林省畜禽品种培育计划项目(2019);吉林省现代农业产业技术示范推广项目(吉牧201904)。

摘　　要：为了探究可能影响毛囊发育的WNT6基因在鹅皮肤毛囊各个生长发育时期是否表达以及毛囊发育状况,试验选择18,35,48,68,88,108日龄6个不同生长时期的卡洛斯鹅和籽鹅,采集背部皮肤,通过组织消化法获得皮肤毛囊并提取总RNA,采用荧光定量PCR的方法计算WNT6基因在皮肤毛囊中的相对表达量,并通过HE染色法观察不同时期毛囊的发育状况及毛囊的不同形态结构。结果表明:WNT6基因在鹅皮肤毛囊的各个生长发育阶段均有表达且表达量有较大差别。在卡洛斯鹅中,WNT6基因在18日龄中相对表达量最高,88日龄相对表达量最低。在籽鹅中,WNT6基因在108日龄中相对表达量最高,88日龄中相对表达量最低。表明WNT6基因在参与鹅的皮肤毛囊生长发育过程中起到一定的促进作用。In order to explore the possible effects of WNT6 genes on hair follicle development,WNT6 genes expressed in goose skin feather follicle and feather follicle development were measured respectively at 18,35,48,68,88 and 108 days of age representing six different growth periods.Carlos goose and Zi goose were used for the study.The back skin was collected,the skin feather follicles were obtained by tissue digestion method and the total RNA was extracted.The relative expression of WNT6 gene in feather follicles of skin was obtained by fluorescence quantitative PCR,and the development status and different morphological structures of feather follicle at different stages were observed by HE staining.The results indicated that WNT6 gene was expressed at different growth and feather development stages of goose skin feather follicles and the expression level was significantly different.Among Carlos geese,the relative expression level of WNT6 gene was the highest at 18 days and the lowest at 88 days.Among Zi geese,the highest relative expression of WNT6 gene was recorded at 108 days and the lowest at 88 days.The results showed that WNT6 gene plays a critical role in promoting the growth and development of skin feather follicles in goose.

关 键 词：WNT6基因 毛囊生长 卡洛斯鹅 籽鹅 

分 类 号：S835[农业科学—畜牧学] Q786[农业科学—畜牧兽医]