香蕉果实MAPs测定方法的建立及其含量变化规律研究  

Establishment of a determination method of MAPs in banana fruit and study on its content variation rule

作　　者：董涛[1] 孙陈康 马兆成 窦同心[1] 高慧君[1] 易干军[1] DONG Tao;SUN Chen-kang;MAZhao-cheng;DOU Tong-xin;GAO Hui-jun;YI Gan-jun(Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences/Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou,Guangdong 510640,China;College of Horticulture and Forestry Sciences,Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology,Ministry of Education,Wuhan,Hubei 430070,China)

机构地区：[1]广东省农业科学院果树研究所/农业农村部南亚热带果树生物学与遗传资源利用重点实验室/广东省热带亚热带果树研究重点实验室,广东广州510640 [2]华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,湖北武汉430070

出　　处：《南方农业学报》2022年第5期1235-1244,共10页Journal of Southern Agriculture

基　　金：国家重点研发计划项目(2019YFD1000903);广东省香蕉菠萝现代农业产业技术体系创新团队项目(2021KJ109)。

摘　　要：【目的】建立一种准确、快速测定香蕉果实单胺类及其前体物质(Monoamines and their precursors,MAPs)的方法,明确香蕉果实发育过程中MAPs各组分的含量变化规律,为下一步解析其合成代谢机理提供参考依据。【方法】以巴西香蕉、粉杂1号粉蕉和东莞大蕉3个香蕉品种的果实为试验材料,采用反相高效液相色谱—紫外检测(RPHPLC-UV)方法测定断蕾后15~75 d的果肉和果皮中MAPs含量,研究其在香蕉果实发育过程中的变化规律。【结果】RP-HPLC-UV色谱条件:采用C_(18)色谱柱(4.6×250 mm,5μm),甲醇为流动相A、0.1%三氟乙酸水溶液为流动相B,定量检测到去甲肾上腺素(NE)、多巴胺(DA)、左旋多巴(L-dopa)、酪胺(TYRA)、酪氨酸(Tyr)、血清素(SERO)、色胺(TRM)和色氨酸(Trp)等8种MAPs含量。其加标回收率为95.3%~106.1%;日内精密度范围为0.37%~0.82%,日间精密度范围为0.35%~0.83%;检测限为0.0012~0.0016 mg/100 g,定量限为0.0040~0.0053 mg/100 g;一个样品在14 min内可以完成检测。3个香蕉品种果实发育过程中,果皮TRM、TYRA、L-dopa、NE和DA含量均高于果肉。东莞大蕉和粉杂1号粉蕉果皮中SERO、Trp和Tyr含量高于果肉,而巴西香蕉果皮中Trp和Tyr含量低于果肉。在果实发育前期,巴西香蕉果肉中大部分MAPs物质的含量有一个快速升高的过程,在断蕾后30 d达峰值,NE、DA、TYRA、Tyr、Trp和TRM含量分别为22.14、239.81、118.29、43.74、46.41和5.33 mg/100 g,之后含量持续下降,NE、DA、TYRA和TRM在果实成熟时达最低值,含量分别为3.17、7.08、12.58和1.04 mg/100 g,而Tyr和Trp含量最低值出现在断蕾后45 d。东莞大蕉和粉杂1号粉蕉中TYRA和SERO含量在果实发育过程中呈下降趋势。【结论】建立的RP-HPLC-UV方法准确、灵敏、快速,可用于香蕉果实中MAPs的定量检测;3个香蕉品种不同发育阶段的果肉和果皮中均可检测到NE、DA、L-dopa、TYRA、Tyr、SERO、TRM和Trp等8种MAPs物质,且其�【Objective】To establish an method for accurate and rapid determination of monoamines and their precursors(MAPs)in banana fruit,to clarify the changes in the content of MAPs components during banana fruit development,so as to provide reference for analyzing its anabolic mechanism in the next step.【Method】Bnana fruits of three banana varieties,Baxi,Dongguan Dajiao and Fenza 1 were taken as tested material.The reversed-phase high performance liquid chromatography-ultraviolet(RP-HPLC-UV)was used to determine the contents of MAPs in pulp and peel of Baxi,Fenza1 and Dongguan Dajiao were detected from 15 to 75 days after cutting bud,to study how MAPs changed during the fruit development.【Result】A C_(18)column(4.6×250 mm,5μm)was connected to the RP-HPLC-UV platform.Methanol and0.1%trifluoroacetic acid aqueous solution were used as mobile phase A and B respectively.Then the contents of norepinephrine(NE),dopamine(DA),levodopa(L-dopa),tyramine(TYRA),tyrosine(Tyr),serotonin(SERO),tryptamine(TRM)and tryptophan(Trp)were determined.The spiked recoveries range was 95.3%to 106.1%,the intra-day precision ranges was 0.37%to 0.82%,and the inter-day precision ranges was 0.35%to 0.83%;the detection limit was 0.0012 to0.0016 mg/100 g,the limit of quantification was 0.0040 to 0.0053 mg/100 g;a sample can be completely detected within14 minutes.During fruit development of the three banana varieties,the contents of TRM,TYRA,L-dopa,NE and DA in the peel were higher than those in the pulp.In addition,the contents of SERO,Trp and Tyr in the peels of Dongguan Dajiao and Fenza 1 were higher than those in the pulp,while the contents of Trp and Tyr in the peels of Baxi were lower than those in the pulp.The contents of most MAPs increased rapidly in the early stage of fruit development in Baxi pulp,reaching the peak around 30 days after cutting bud,the contents of NE,DA,TYRA,Tyr,Trp and TRM were 22.14,239.81,118.29,43.74,46.41 and 5.33 mg/100 g.After that,the contents of NE,DA,TYRA and TRM reached the lowest point when the fruit ma

关 键 词：香蕉 果实 发育过程 单胺类及其前体物质 反相高效液相色谱—紫外检测 

分 类 号：S668.1[农业科学—果树学]